PDBsum entry 2wly

Hydrolase

PDB id: 2wly

Contents

Protein chain

539 a.a. *

Ligands

DIO ×5

PEG ×3

SN5-NGT

Waters ×326

* Residue conservation analysis

PDB id:

2wly

Name:

Hydrolase

Title:

Chitinase a from serratia marcescens atcc990 in complex with chitotrio-thiazoline.

Structure:

Chitinase. Chain: a. Fragment: residues 2-520. Engineered: yes

Source:

Serratia marcescens. Organism_taxid: 615. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.40Å R-factor: 0.158 R-free: 0.205

Authors:

E.J.Taylor,R.J.Dennis,J.M.Macdonald,C.A.Tarling,S.Knapp,S.G.Withers, G.J.Davies

Key ref:

J.M.Macdonald et al. (2010). Chitinase inhibition by chitobiose and chitotriose thiazolines. Angew Chem Int Ed Engl, 49, 2599-2602. PubMed id: 20209544

Date:

29-Jun-09 Release date: 16-Mar-10

Protein chain

P07254  (CHIA_SERMA) -  Chitinase A from Serratia marcescens

Seq: 563 a.a.

Struc: 539 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.14 - chitinase.
• Reaction: Hydrolysis of the 1,4-beta-linkages of N-acetyl-D-glucosamine polymers of chitin.

Angew Chem Int Ed Engl 49:2599-2602 (2010)

PubMed id: 20209544

Chitinase inhibition by chitobiose and chitotriose thiazolines.

J.M.Macdonald, C.A.Tarling, E.J.Taylor, R.J.Dennis, D.S.Myers, S.Knapp, G.J.Davies, S.G.Withers.

ABSTRACT

No abstract given.