spacer
spacer

PDBsum entry 2wj8

Go to PDB code: 
Top Page protein dna_rna ligands Protein-protein interface(s) links
RNA binding protein/RNA PDB id
2wj8
Jmol
Contents
Protein chains
(+ 14 more) 374 a.a.
DNA/RNA
Ligands
BO4 ×12
Waters ×685

References listed in PDB file
Key reference
Title The 24-Angstrom structure of respiratory syncytial virus nucleocapsid protein-Rna decameric rings.
Authors K.Maclellan, C.Loney, R.P.Yeo, D.Bhella.
Ref. J Virol, 2007, 81, 9519-9524.
PubMed id 17567697
Abstract
Respiratory syncytial virus (RSV), a nonsegmented, negative-sense RNA-containing virus, is a common cause of lower respiratory tract disease. Expression of RSV nucleocapsid protein (N) in insect cells using the baculovirus expression system leads to the formation of N-RNA complexes that are morphologically indistinguishable from viral nucleocapsids. When imaged in an electron microscope, three distinct types of structures were observed: tightly wound short-pitch helices, highly extended helices, and rings. Negative stain images of N-RNA rings were used to calculate a three-dimensional reconstruction at 24 A resolution, revealing features similar to those observed in nucleocapsids from other viruses of the order Mononegavirales. The reconstructed N-RNA rings comprise 10 N monomers and have an external radius of 83 A and an internal radius of 40 A. Comparison of this structure with crystallographic data from rabies virus and vesicular stomatitis virus N-RNA rings reveals striking morphological similarities.
Secondary reference #1
Title The 24-Angstrom structure of respiratory syncytial virus nucleocapsid protein-Rna decameric rings.
Authors K.Maclellan, C.Loney, R.P.Yeo, D.Bhella.
Ref. J Virol, 2007, 81, 9519-9524.
PubMed id 17567697
Abstract
PROCHECK
Go to PROCHECK summary
 Headers