PDBsum entry 2wbs

Transcription/DNA

PDB id: 2wbs

Contents

Protein chain

87 a.a. *

DNA/RNA

Ligands

GOL

Metals

_ZN ×3

Waters ×131

* Residue conservation analysis

PDB id:

2wbs

Name:

Transcription/DNA

Title:

Crystal structure of the zinc finger domain of klf4 bound to its target DNA

Structure:

Krueppel-like factor 4. Chain: a. Fragment: DNA-binding domain, residues 395-483. Synonym: gut-enriched krueppel-like factor, epithelial zinc finger protein ezf. Engineered: yes. 5'-d( Gp Ap Gp Gp Cp Gp Cp)-3'. Chain: f. Synonym: heptameric DNA binding sequence of klf4.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Strain: c57bl/6. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. House mouse. Organism_taxid: 10090

Resolution:

1.70Å R-factor: 0.202 R-free: 0.232

Authors:

G.Zocher,A.Schuetz,D.Carstanjen,U.Heinemann

Key ref:

A.Schuetz et al. (2011). The structure of the Klf4 DNA-binding domain links to self-renewal and macrophage differentiation. Cell Mol Life Sci, 68, 3121-3131. PubMed id: 21290164

Date:

03-Mar-09 Release date: 07-Apr-10

Protein chain

Q60793  (KLF4_MOUSE) -  Krueppel-like factor 4 from Mus musculus

Seq: 483 a.a.

Struc: 87 a.a.

DNA/RNA chains

G-A-G-G-C-G-C 7 bases

G-C-G-C-C-T-C 7 bases

Enzyme reactions

• Enzyme class: E.C.?

Cell Mol Life Sci 68:3121-3131 (2011)

PubMed id: 21290164

The structure of the Klf4 DNA-binding domain links to self-renewal and macrophage differentiation.

A.Schuetz, D.Nana, C.Rose, G.Zocher, M.Milanovic, J.Koenigsmann, R.Blasig, U.Heinemann, D.Carstanjen.

ABSTRACT

No abstract given.