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UniProt functional annotation for Q9UBT6 | ||
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| UniProt code: Q9UBT6. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. {ECO:0000269|PubMed:10620008, ECO:0000269|PubMed:11024016, ECO:0000269|PubMed:12145297, ECO:0000269|PubMed:12444249, ECO:0000269|PubMed:12952891, ECO:0000269|PubMed:14630940, ECO:0000269|PubMed:15533436, ECO:0000269|PubMed:28297716}. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; Evidence={ECO:0000269|PubMed:11024016}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11024016}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:11024016}; Note=Divalent metal cations. Prefers Mg(2+), but can also use Mn(2+). {ECO:0000269|PubMed:11024016}; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.5-7.5. {ECO:0000269|PubMed:11024016}; Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:11024016}; |
| Subunit: | |
Interacts with REV1 (By similarity). Interacts with PCNA (PubMed:11784855). {ECO:0000250|UniProtKB:Q9QUG2, ECO:0000269|PubMed:11784855}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:12414988, ECO:0000269|PubMed:28297716}. Note=Detected throughout the nucleus and at replication foci (PubMed:12414988). Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)- containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716). {ECO:0000269|PubMed:12414988, ECO:0000269|PubMed:28297716}. |
| Tissue specificity: | |
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. {ECO:0000269|PubMed:10518552, ECO:0000269|PubMed:10620008}. |
| Domain: | |
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. |
| Miscellaneous: | |
[Isoform 4]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. {ECO:0000305}. |
| Similarity: | |
Belongs to the DNA polymerase type-Y family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.