UniProt functional annotation for A5HAK0



	UniProt functional annotation for A5HAK0

UniProt code: A5HAK0.

Organism: Danio rerio (Zebrafish) (Brachydanio rerio).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio.

Function: Ribonuclease. Angiogenic. Plays a role in host defense. Exhibits strong antibacterial activity against Gram-negative bacteria but mild antibacterial activity against Gram-positive bacteria. The RNase activity is not required for the bactericidal activity. {ECO:0000269|PubMed:16861230, ECO:0000269|PubMed:17347156, ECO:0000269|PubMed:18508078, ECO:0000269|PubMed:20214681}.

Subcellular location: Secreted {ECO:0000250|UniProtKB:P34096}.

Tissue specificity: Strongly expressed in the adult liver and gut, and weakly in the heart and testis. {ECO:0000269|PubMed:17347156}.

Developmental stage: Only expressed in adults. {ECO:0000269|PubMed:17347156}.

Ptm: Cleavage between Arg-55 and Arg-56 is catalyzed by a membrane- localized Gram-negative bacterium protease (OmpT in E.coli). The excised fragment is then transported to the bacterium cytosol for cleavage of the disulfide bridge linking Cys-48 and Cys-109, thus separating the N-terminal and LF-ZF3. LF-ZF3 but not the N-terminal peptide possesses bactericidal activity. {ECO:0000269|PubMed:20214681}.

Miscellaneous: Although the OmpT protease is absent in Gram-positive bacteria, LF-ZF3 generated by a Gram-negative bacterium can penetrate a Gram-positive bacterium and exert its cytotoxicity. {ECO:0000269|PubMed:20214681}.

Similarity: Belongs to the pancreatic ribonuclease family. {ECO:0000255}.

Annotations taken from UniProtKB at the EBI.


Organism:		Danio rerio (Zebrafish) (Brachydanio rerio).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio.



Function:		Ribonuclease. Angiogenic. Plays a role in host defense. Exhibits strong antibacterial activity against Gram-negative bacteria but mild antibacterial activity against Gram-positive bacteria. The RNase activity is not required for the bactericidal activity. {ECO:0000269\|PubMed:16861230, ECO:0000269\|PubMed:17347156, ECO:0000269\|PubMed:18508078, ECO:0000269\|PubMed:20214681}.


Subcellular location:		Secreted {ECO:0000250\|UniProtKB:P34096}.
Tissue specificity:		Strongly expressed in the adult liver and gut, and weakly in the heart and testis. {ECO:0000269\|PubMed:17347156}.
Developmental stage:		Only expressed in adults. {ECO:0000269\|PubMed:17347156}.
Ptm:		Cleavage between Arg-55 and Arg-56 is catalyzed by a membrane- localized Gram-negative bacterium protease (OmpT in E.coli). The excised fragment is then transported to the bacterium cytosol for cleavage of the disulfide bridge linking Cys-48 and Cys-109, thus separating the N-terminal and LF-ZF3. LF-ZF3 but not the N-terminal peptide possesses bactericidal activity. {ECO:0000269\|PubMed:20214681}.
Miscellaneous:		Although the OmpT protease is absent in Gram-positive bacteria, LF-ZF3 generated by a Gram-negative bacterium can penetrate a Gram-positive bacterium and exert its cytotoxicity. {ECO:0000269\|PubMed:20214681}.
Similarity:		Belongs to the pancreatic ribonuclease family. {ECO:0000255}.