PDBsum entry 2vq1

Immune system

PDB id

2vq1

Loading ...

Contents

			Protein chains

					217 a.a.


					213 a.a. *

			Ligands

					GLY ×8


					GOL ×3


					AZI

			Waters ×214

* Residue conservation analysis

PDB id:

2vq1

Links

Name:

Immune system

Title:

Anti trimeric lewis x fab54-5c10-a

Structure:

Igkv1-117 protein. Chain: a, e. Synonym: fab54-5c10-a. Other_details: antigen binding fragment. Anti-human fc gamma receptor iii 3g8 gamma heavy chain variable region. Chain: b, f. Synonym: fab54-5c10-a. Other_details: antigen binding fragment

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Strain: swiss. Cell_line: murine hybridoma. Cell_line: murine hybridoma

Resolution:

2.50Å

R-factor:

0.206

R-free:

0.260

Authors:

D.C.De Geus,A.M.M.Van Roon,E.A.J.Thomassen,C.H.Hokke,A.M.Deelder, J.P.Abrahams

Key ref:

D.C.de Geus et al. (2009). Characterization of a diagnostic Fab fragment binding trimeric Lewis X. Proteins, 76, 439-447. PubMed id: 19173313

Date:

10-Mar-08

Release date:

27-Jan-09

PROCHECK

	Headers

	References

Protein chains

No UniProt id for this chain

Struc:					217 a.a.

Protein chains

No UniProt id for this chain

Struc:					213 a.a.

Key:

Secondary structure

CATH domain

	Proteins 76:439-447 (2009)
PubMed id: 19173313

Characterization of a diagnostic Fab fragment binding trimeric Lewis X.
D.C.de Geus, A.M.van Roon, E.A.Thomassen, C.H.Hokke, A.M.Deelder, J.P.Abrahams.

ABSTRACT

Lewis X trisaccharides normally function as essential cell-cell interaction mediators. However, oligomers of Lewis X trisaccharides expressed by the parasite Schistosoma mansoni seem to be related to its evasion of the immune response of its human host. Here we show that monoclonal antibody 54-5C10-A, which is used to diagnose schistosomiasis in humans, interacts with oligomers of at least three Lewis X trisaccharides, but not with monomeric Lewis X. We describe the sequence and the 2.5 A crystal structure of its Fab fragment and infer a possible mode of binding of the polymeric Lewis X from docking studies. Our studies indicate a radically different mode of binding compared to Fab 291-2G3-A, which is specific for monomeric Lewis X, thus providing a structural explanation of the diagnostic success of 54-5C10-A.