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PDBsum entry 2vlr
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Immune system
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PDB id
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2vlr
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Contents |
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276 a.a.
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100 a.a.
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199 a.a.
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240 a.a.
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References listed in PDB file
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Key reference
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Title
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The structural dynamics and energetics of an immunodominant t cell receptor are programmed by its vbeta domain.
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Authors
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J.Ishizuka,
G.B.Stewart-Jones,
A.Van der merwe,
J.I.Bell,
A.J.Mcmichael,
E.Y.Jones.
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Ref.
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Immunity, 2008,
28,
171-182.
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PubMed id
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Abstract
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Immunodominant and public T cell receptor (TCR) usage is relatively common in
many viral diseases yet surprising in the context of the large naive TCR
repertoire. We examined the highly conserved Vbeta17:Valpha10.2 JM22 T cell
response to the influenza matrix peptide (58-66)-HLA-A*0201 (HLA-A2-flu) through
extensive kinetic, thermodynamic, and structural analyses. We found several
conformational adjustments that accompany JM22-HLA-A2-flu binding and identified
a binding "hotspot" within the Vbeta domain of the TCR. Within this hotspot, key
germline-encoded CDR1 and CDR2 loop residues and a crucial but commonly coded
residue in the hypervariable region of CDR3 provide the basis for the
substantial bias in the selection of the germline-encoded Vbeta17 domain. The
chances of having a substantial number of T cells in the naive repertoire that
have HLA-A2-flu-specific Vbeta17 receptors may consequently be relatively high,
thus explaining the immunodominant usage of this clonotype.
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