PDBsum entry 2vkn

Membrane protein

PDB id: 2vkn

Contents

Protein chain

66 a.a. *

Ligands

LYS-PRO-LEU-PRO-PRO-LEU-PRO-LEU-ALA

SO4 ×3

Waters ×37

* Residue conservation analysis

PDB id:

2vkn

Name:

Membrane protein

Title:

Yeast sho1 sh3 domain complexed with a peptide from pbs2

Structure:

Protein ssu81. Chain: a. Fragment: sh3 domain, residues 298-367. Synonym: sho1 osmosensor, sho1. Engineered: yes. Map kinase kinase pbs2. Chain: c. Fragment: proline-rich domain, residues 2-13. Synonym: polymyxin b resistance protein 2, suppressor of fluoride

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 4932

Resolution:

2.05Å R-factor: 0.171 R-free: 0.238

Authors:

P.Kursula,I.Kursula,Y.H.Song,K.Paraskevopoulos,M.Wilmanns

Key ref:

P.Kursula et al. Structural genomics of yeast sh3 domains. To be published, .

Date:

20-Dec-07 Release date: 05-Feb-08

Supersedes:

2qk6

Protein chain

P40073  (SHO1_YEAST) -  High osmolarity signaling protein SHO1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 367 a.a.

Struc: 66 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.12.2 - mitogen-activated protein kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
  3. L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site