PDBsum entry 2vir

Complex (hemagglutinin/immunoglobulin)

PDB id

2vir

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Contents

			Protein chains

					210 a.a. *


					221 a.a. *


					267 a.a. *

			Metals

					_ZN ×3

* Residue conservation analysis

PDB id:

2vir

Links

Name:

Complex (hemagglutinin/immunoglobulin)

Title:

Influenza virus hemagglutinin complexed with a neutralizing antibody

Structure:

Immunoglobulin (igg1, lambda). Chain: a. Fragment: fab fragment. Immunoglobulin (igg1, lambda). Chain: b. Fragment: fab fragment. Hemagglutinin. Chain: c. Fragment: proteolytic fragment "ha top" containing ha1 residues 28 -

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Strain: balb/c. Cell: hybridoma. Strain: balb-c. Influenza a virus (a/x-31(h3n2)). Organism_taxid: 132504. Strain: x31.

Biol. unit:

Homo-Trimer (from PDB file)

Resolution:

3.25Å

R-factor:

0.198

R-free:

0.249

Authors:

T.Bizebard,D.Fleury,B.Gigant,S.A.Wharton,J.J.Skehel,M.Knossow

Key ref:

D.Fleury et al. (1998). Antigen distortion allows influenza virus to escape neutralization. Nat Struct Biol, 5, 119-123. PubMed id: 9461077

Date:

22-Dec-97

Release date:

29-Apr-98

Supersedes:

1vir

PROCHECK

	Headers

	References

Protein chain

No UniProt id for this chain

Struc:					210 a.a.

Protein chain

Q99LC4 (Q99LC4_MOUSE) - Igh protein from Mus musculus

Seq: Struc:					463 a.a. 221 a.a.*

Protein chain

P03437 (HEMA_I68A0) - Hemagglutinin from Influenza A virus (strain A/Aichi/2/1968 H3N2)

Seq: Struc:

Seq: Struc:					566 a.a. 267 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 71 residue positions (black crosses)

	Nat Struct Biol 5:119-123 (1998)
PubMed id: 9461077

Antigen distortion allows influenza virus to escape neutralization.
D.Fleury, S.A.Wharton, J.J.Skehel, M.Knossow, T.Bizebard.

ABSTRACT

The structure of the hemagglutinin (HA) of a mutant influenza virus that escapes neutralization by a monoclonal antibody shows that the mutation causes changes in HA structure which avoid an energetically less favorable conformation. However, the structure of the mutant HA.Fab complex indicates that the antibody binds selectively to mutant HA in a wild type-like distorted conformation. The association of an antibody with a less favored HA conformation represents an alternative to previously described mechanisms of escape from neutralization by antibodies.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21213247

P.L.Kastritis, I.H.Moal, H.Hwang, Z.Weng, P.A.Bates, A.M.Bonvin, and J.Janin (2011).
A structure-based benchmark for protein-protein binding affinity.

Protein Sci, 20, 482-491.

21251008

T.Han, and W.A.Marasco (2011).
Structural basis of influenza virus neutralization.

Ann N Y Acad Sci, 1217, 178-190.

20232097

A.Malik, A.Firoz, V.Jha, E.Sunderasan, and S.Ahmad (2010).
Modeling the three-dimensional structures of an unbound single-chain variable fragment (scFv) and its hypothetical complex with a Corynespora cassiicola toxin, cassiicolin.

J Mol Model, 16, 1883-1893.

20038580

A.Yokota, K.Tsumoto, M.Shiroishi, T.Nakanishi, H.Kondo, and I.Kumagai (2010).
Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme.

J Biol Chem, 285, 7686-7696.

PDB codes:

3a67 3a6b 3a6c

20388627

W.D.Lees, D.S.Moss, and A.J.Shepherd (2010).
A computational analysis of the antigenic properties of haemagglutinin in influenza A H3N2.

Bioinformatics, 26, 1403-1408.

20602265

Y.Sun, Y.Shi, W.Zhang, Q.Li, D.Liu, C.Vavricka, J.Yan, and G.F.Gao (2010).
In silico characterization of the functional and structural modules of the hemagglutinin protein from the swine-origin influenza virus A (H1N1)-2009.

Sci China Life Sci, 53, 633-642.

19251591

D.C.Ekiert, G.Bhabha, M.A.Elsliger, R.H.Friesen, M.Jongeneelen, M.Throsby, J.Goudsmit, and I.A.Wilson (2009).
Antibody recognition of a highly conserved influenza virus epitope.

Science, 324, 246-251.

PDB codes:

3gbm 3gbn

17996091

B.D.Silverman (2007).
Using molecular principal axes for structural comparison: determining the tertiary changes of a FAB antibody domain induced by antigenic binding.

BMC Struct Biol, 7, 77.

17166830

M.Shiroishi, K.Tsumoto, Y.Tanaka, A.Yokota, T.Nakanishi, H.Kondo, and I.Kumagai (2007).
Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex. The case of HyHEL-10-HEL.

J Biol Chem, 282, 6783-6791.

PDB codes:

2dqc 2dqd 2dqe 2dqf 2dqg 2dqh 2dqi 2dqj

17040574

B.J.Hanson, A.C.Boon, A.P.Lim, A.Webb, E.E.Ooi, and R.J.Webby (2006).
Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice.

Respir Res, 7, 126.

16925526

M.Knossow, and J.J.Skehel (2006).
Variation and infectivity neutralization in influenza.

Immunology, 119, 1-7.

16252003

J.Yan, W.Wen, W.Xu, J.F.Long, M.E.Adams, S.C.Froehner, and M.Zhang (2005).
Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin.

EMBO J, 24, 3985-3995.

PDB codes:

1z86 1z87 2adz

15858030

K.Nakajima, E.Nobusawa, A.Nagy, and S.Nakajima (2005).
Accumulation of amino acid substitutions promotes irreversible structural changes in the hemagglutinin of human influenza AH3 virus during evolution.

J Virol, 79, 6472-6477.

12709438

I.Kumagai, Y.Nishimiya, H.Kondo, and K.Tsumoto (2003).
Structural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10.

J Biol Chem, 278, 24929-24936.

PDB codes:

1ua6 1uac

11517321

I.B.Rasmussen, E.Lunde, T.E.Michaelsen, B.Bogen, and I.Sandlie (2001).
The principle of delivery of T cell epitopes to antigen-presenting cells applied to peptides from influenza virus, ovalbumin, and hen egg lysozyme: implications for peptide vaccination.

Proc Natl Acad Sci U S A, 98, 10296-10301.

10899782

D.Fleury, R.S.Daniels, J.J.Skehel, M.Knossow, and T.Bizebard (2000).
Structural evidence for recognition of a single epitope by two distinct antibodies.

Proteins, 40, 572-578.

PDB code:

1eo8

10966468

J.J.Skehel, and D.C.Wiley (2000).
Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.

Annu Rev Biochem, 69, 531-569.

10451557

B.Selisko, A.F.Licea, B.Becerril, F.Zamudio, L.D.Possani, and E.Horjales (1999).
Antibody BCF2 against scorpion toxin Cn2 from Centuroides noxius Hoffmann: primary structure and three-dimensional model as free Fv fragment and complexed with its antigen.

Proteins, 37, 130-143.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.