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UniProt functional annotation for Q12009 | ||
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| UniProt code: Q12009. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Methyltransferase that catalyzes the formation of N(7)- methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity. {ECO:0000255|HAMAP-Rule:MF_03055, ECO:0000269|PubMed:16387656, ECO:0000269|PubMed:17382321}. |
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| Catalytic activity: | |
Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74480; EC=2.1.1.33; Evidence={ECO:0000255|HAMAP- Rule:MF_03055, ECO:0000269|PubMed:15811913}; |
| Pathway: | |
tRNA modification; N(7)-methylguanine-tRNA biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03055}. |
| Subunit: | |
Forms a complex with TRM82. {ECO:0000255|HAMAP-Rule:MF_03055, ECO:0000269|PubMed:18184583}. |
| Subcellular location: | |
Nucleus {ECO:0000255|HAMAP-Rule:MF_03055, ECO:0000269|PubMed:14562095}. |
| Miscellaneous: | |
Present with 2630 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. {ECO:0000255|HAMAP-Rule:MF_03055}. |
Annotations taken from UniProtKB at the EBI.