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UniProt functional annotation for P0DJQ5 | ||
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| UniProt codes: P0DJQ5, Q53940. |
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| Organism: | |
Streptomyces clavuligerus. |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; Streptomyces. |
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| Function: | |
Catalyzes the biosynthesis of ornithine by transacetylation between N(2)-acetylornithine and glutamate. It can also use L-arginine, L-glutamine and L-lysine as acetyl acceptors. {ECO:0000269|PubMed:11985581}. |
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| Catalytic activity: | |
Reaction=L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N- acetyl-L-glutamate; Xref=Rhea:RHEA:15349, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:46911, ChEBI:CHEBI:57805; EC=2.3.1.35; Evidence={ECO:0000269|PubMed:11985581}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.6 mM for L-N-acetylornithine (at 37 degrees Celsius and pH 8.0) {ECO:0000269|PubMed:11985581}; Vmax=87 nmol/min/mg enzyme {ECO:0000269|PubMed:11985581}; pH dependence: Optimum pH is between 7.5-8. {ECO:0000269|PubMed:11985581}; |
| Pathway: | |
Antibiotic biosynthesis; clavulanate biosynthesis. |
| Subunit: | |
Heterotetramer of two alpha and two beta chains. {ECO:0000269|PubMed:11985581, ECO:0000269|PubMed:15352873, ECO:0000269|PubMed:19105697, ECO:0000269|PubMed:21796301, ECO:0000269|Ref.4}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Mass spectrometry: | |
[Glutamate N-acetyltransferase 2 alpha chain]: Mass=18816.2; Method=Electrospray; Evidence={ECO:0000269|PubMed:11985581}; |
| Mass spectrometry: | |
[Glutamate N-acetyltransferase 2 beta chain]: Mass=22811.7; Method=Electrospray; Evidence={ECO:0000269|PubMed:11985581}; |
| Miscellaneous: | |
The role of this protein is probably directed towards producing increased intracellular concentrations of arginine for clavam biosynthesis, rather than primary metabolism. {ECO:0000305|PubMed:11985581}. |
| Similarity: | |
Belongs to the ArgJ family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.