PDBsum entry 2v02

Metal binding protein

PDB id

2v02

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Contents

			Protein chain

					142 a.a. *

			Metals

					_BA ×2


					_CA ×3

			Waters ×8

* Residue conservation analysis

PDB id:

2v02

Links

Name:

Metal binding protein

Title:

Recombinant vertebrate calmodulin complexed with ba

Structure:

Calmodulin. Chain: a. Synonym: cam. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta plyss.

Resolution:

2.20Å

R-factor:

0.246

R-free:

0.294

Authors:

P.Kursula,V.Majava

Key ref:

P.Kursula and V.Majava (2007). A structural insight into lead neurotoxicity and calmodulin activation by heavy metals. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 653-656. PubMed id: 17671360 DOI: 10.1107/S1744309107034525

Date:

04-May-07

Release date:

14-Aug-07

PROCHECK

	Headers

	References

Protein chain

P0DP23 (CALM1_HUMAN) - Calmodulin-1 from Homo sapiens

Seq: Struc:					149 a.a. 142 a.a.

Key:

PfamA domain

Secondary structure

DOI no: 10.1107/S1744309107034525	Acta Crystallogr Sect F Struct Biol Cryst Commun 63:653-656 (2007)
PubMed id: 17671360

A structural insight into lead neurotoxicity and calmodulin activation by heavy metals.
P.Kursula, V.Majava.

ABSTRACT

Calmodulin is a calcium sensor that is also capable of binding and being activated by other metal ions. Of specific interest in this respect is lead, which is known to be neurotoxic and to have a very high affinity towards calmodulin. Crystal structures of human calmodulin complexed with lead and barium ions have been solved. The results will help in understanding the activation mechanisms of calmodulin by different heavy metals and will provide a detailed view of a putative target for lead neurotoxicity in humans.

Selected figure(s)



	Figure 1. Stereoviews of the overall structures of the complexes and the locations of the bound heavy-metal atoms: (a) the Pb complex, (b) the Ba complex. Small green spheres indicate Ca atoms, large magenta spheres indicate soaked heavy-metal atoms bound to the EF-hands and yellow large spheres indicate heavy-metal atoms bound outside the EF-hands. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 August 1; 63(Pt 8): 653–656. Published online 2007 July 28. doi: 10.1107/S1744309107034525. Copyright [copyright] International Union of Crystallography 2007		Figure 2. Anomalous difference map (cyan) around the bound Ba atoms (magenta) at EF-hand 2 contoured at 5[sigma]. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 August 1; 63(Pt 8): 653–656. Published online 2007 July 28. doi: 10.1107/S1744309107034525. Copyright [copyright] International Union of Crystallography 2007

Literature references that cite this PDB file's key reference

PubMed id

Reference

19404396

V.Majava, and P.Kursula (2009).
Domain swapping and different oligomeric States for the complex between calmodulin and the calmodulin-binding domain of calcineurin a.

PLoS ONE, 4, e5402.

PDB code:

2w73

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