PDBsum entry 2uzp

Transferase

PDB id: 2uzp

Contents

Protein chain

142 a.a. *

Ligands

PLP ×3

SO4 ×20

EDO ×8

Metals

_CO ×3

_CA ×9

Waters ×392

* Residue conservation analysis

PDB id:

2uzp

Name:

Transferase

Title:

Crystal structure of the c2 domain of human protein kinasE C gamma.

Structure:

Protein kinasE C gamma type. Chain: a, b, c. Fragment: c2 domain, residues 154-295. Synonym: protein kinasE C gamma, pkc-gamma. Engineered: yes. Other_details: schiffs base link between ser -1 and pyridoxal phosphate plp300

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.172 R-free: 0.205

Authors:

A.C.W.Pike,A.Amos,C.Johansson,F.Sobott,P.Savitsky,G.Berridge, O.Fedorov,C.Umeano,F.Gorrec,G.Bunkoczi,J.Debreczeni,F.Von Delft, C.H.Arrowsmith,A.Edwards,J.Weigelt,M.Sundstrom,S.Knapp

Key ref:

A.C.W.Pike et al. Crystal structure of c2 domain of protein kinase c gamma. To be published, .

Date:

30-Apr-07 Release date: 29-May-07

Protein chains

P05129  (KPCG_HUMAN) -  Protein kinase C gamma type from Homo sapiens

Seq: 697 a.a.

Struc: 142 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.13 - protein kinase C.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = PLP) matches with 44.83% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = PLP) matches with 44.83% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site