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UniProt functional annotation for P32169 | ||
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| UniProt code: P32169. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the reversible cleavage of L-rhamnulose-1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde (PubMed:12962479). Also catalyzes the dephosphorylation of phospho- serine in vitro (PubMed:25848029). {ECO:0000269|PubMed:12962479, ECO:0000269|PubMed:25848029}. |
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| Catalytic activity: | |
Reaction=L-rhamnulose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate; Xref=Rhea:RHEA:19689, ChEBI:CHEBI:18041, ChEBI:CHEBI:57642, ChEBI:CHEBI:58313; EC=4.1.2.19; Evidence={ECO:0000269|PubMed:12962479}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:11976494, ECO:0000269|PubMed:12962479}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:11976494, ECO:0000269|PubMed:12962479}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.48 mM for L-rhamnulose 1-phosphate (at 37 degrees Celsius) {ECO:0000269|PubMed:12962479}; |
| Pathway: | |
Carbohydrate degradation; L-rhamnose degradation; glycerone phosphate from L-rhamnose: step 3/3. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:11976494}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
Induced by L-rhamnose via the RhaR-RhaS regulatory cascade. Binding of the cAMP receptor protein (CRP) is required for full expression. {ECO:0000269|PubMed:10852886, ECO:0000269|PubMed:8230210}. |
| Similarity: | |
Belongs to the aldolase class II family. RhaD subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.