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PDBsum entry 2tld
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Hydrolase/hydrolase inhibitor
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PDB id
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2tld
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References listed in PDB file
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Key reference
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Title
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Crystal structure of an engineered subtilisin inhibitor complexed with bovine trypsin.
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Authors
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Y.Takeuchi,
T.Nonaka,
K.T.Nakamura,
S.Kojima,
K.Miura,
Y.Mitsui.
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Ref.
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Proc Natl Acad Sci U S A, 1992,
89,
4407-4411.
[DOI no: ]
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PubMed id
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Abstract
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Proteinase specificity of a proteinaceous inhibitor of subtilisin (SSI;
Streptomyces subtilisin inhibitor) can be altered so as to strongly inhibit
trypsin simply by replacing P1 methionine with lysine (with or without
concomitant change of the P4 residue) through site-directed mutagenesis. Now the
crystal structure of one such engineered SSI (P1 methionine converted to lysine
and P4 methionine converted to glycine) complexed with bovine trypsin has been
solved at 2.6 A resolution and refined to a crystallographic R factor of 0.173.
Comparing this structure with the previously established structure of the native
SSI complexed with subtilisin BPN', it was found that (i) P1 lysine of the
mutant SSI is accommodated in the S1 pocket of trypsin as usual, and (ii) upon
complex formation, considerable conformation change occurs to the reactive site
loop of the mutant SSI. Thus, in this case, flexibility of the reactive site
loop seems important for successfully changing the proteinase specificity
through mere replacement of the P1 residue.
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Secondary reference #1
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Title
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Refined crystal structure of the complex of subtilisin bpn' And streptomyces subtilisin inhibitor at 1.8 a resolution.
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Authors
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Y.Takeuchi,
Y.Satow,
K.T.Nakamura,
Y.Mitsui.
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Ref.
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J Mol Biol, 1991,
221,
309-325.
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PubMed id
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Headers
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