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PDBsum entry 2tgf
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Growth factor
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PDB id
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2tgf
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References listed in PDB file
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Key reference
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Title
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The solution structure of human transforming growth factor alpha.
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Authors
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T.S.Harvey,
A.J.Wilkinson,
M.J.Tappin,
R.M.Cooke,
I.D.Campbell.
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Ref.
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Eur J Biochem, 1991,
198,
555-562.
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PubMed id
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Abstract
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The solution structure of transforming growth factor alpha has been determined
by a combination of high-resolution 1H-nuclear magnetic resonance and distance
geometry and restrained molecular dynamics. The 382 restraints derived from the
NMR experiments were used to calculate many distance geometry structures, which
were then refined by restrained molecular mechanics. Five of these structures
were further refined using a variety of methods. Comparison of independently
measured parameters, such as calculated hydrogen bonding patterns and
experimental amide exchange rates, have been used to evaluate the accuracy of
the structures. Also, possible mechanisms to explain the pH-dependent
conformational interconversion observed are suggested. Finally comparisons
between this work and others on this topic have been made.
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