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PDBsum entry 2ruz
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Transcription
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PDB id
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2ruz
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References listed in PDB file
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Key reference
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Title
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Solution structures of the DNA-Binding domains of immune-Related zinc-Finger protein zfat.
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Authors
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N.Tochio,
T.Umehara,
K.Nakabayashi,
M.Yoneyama,
K.Tsuda,
M.Shirouzu,
S.Koshiba,
S.Watanabe,
T.Kigawa,
T.Sasazuki,
S.Shirasawa,
S.Yokoyama.
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Ref.
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J Struct Funct Genomics, 2015,
16,
55-65.
[DOI no: ]
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PubMed id
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Abstract
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ZFAT is a transcriptional regulator, containing eighteen C2H2-type zinc-fingers
and one AT-hook, involved in autoimmune thyroid disease, apoptosis, and
immune-related cell survival. We determined the solution structures of the
thirteen individual ZFAT zinc-fingers (ZF) and the tandemly arrayed zinc-fingers
in the regions from ZF2 to ZF5, by NMR spectroscopy. ZFAT has eight uncommon
bulged-out helix-containing zinc-fingers, and six of their structures (ZF4, ZF5,
ZF6, ZF10, ZF11, and ZF13) were determined. The distribution patterns of the
putative DNA-binding surface residues are different among the ZFAT zinc-fingers,
suggesting the distinct DNA sequence preferences of the N-terminal and
C-terminal zinc-fingers. Since ZFAT has three to five consecutive tandem
zinc-fingers, which may cooperatively function as a unit, we also determined two
tandemly arrayed zinc-finger structures, between ZF2 to ZF4 and ZF3 to ZF5. Our
NMR spectroscopic analysis detected the interaction between ZF4 and ZF5, which
are connected by an uncommon linker sequence, KKIK. The ZF4-ZF5 linker
restrained the relative structural space between the two zinc-fingers in
solution, unlike the other linker regions with determined structures, suggesting
the involvement of the ZF4-ZF5 interfinger linker in the regulation of ZFAT
function.
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