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PDBsum entry 2rpq
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Transcription
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PDB id
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2rpq
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References listed in PDB file
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Key reference
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Title
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Structure of the small ubiquitin-Like modifier (sumo)-Interacting motif of mbd1-Containing chromatin-Associated factor 1 bound to sumo-3.
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Authors
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N.Sekiyama,
T.Ikegami,
T.Yamane,
M.Ikeguchi,
Y.Uchimura,
D.Baba,
M.Ariyoshi,
H.Tochio,
H.Saitoh,
M.Shirakawa.
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Ref.
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J Biol Chem, 2008,
283,
35966-35975.
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PubMed id
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Abstract
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Post-translational modification by small ubiquitin-like modifier (SUMO) proteins
has been implicated in the regulation of a variety of cellular events. The
functions of sumoylation are often mediated by downstream effector proteins
harboring SUMO-interacting motifs (SIMs) that are composed of a hydrophobic core
and a stretch of acidic residues. MBD1-containing chromatin-associated factor 1
(MCAF1), a transcription repressor, interacts with SUMO-2/3 and SUMO-1, with a
preference for SUMO-2/3. We used NMR spectroscopy to solve the solution
structure of the SIM of MCAF1 bound to SUMO-3. The hydrophobic core of the SIM
forms a parallel beta-sheet pairing with strand beta2 of SUMO-3, whereas its
C-terminal acidic stretch seems to mediate electrostatic interactions with a
surface area formed by basic residues of SUMO-3. The significance of these
electrostatic interactions was shown by mutations of both SUMO-3 and MCAF1. The
present structural and biochemical data suggest that the acidic stretch of the
SIM of MCAF1 plays an important role in the binding to SUMO-3.
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