UniProt functional annotation for Q75I93

UniProt codes: Q75I93, Q42975.

Organism: Oryza sativa subsp. japonica (Rice).
Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
 
Function: Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity. {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}.
 
Catalytic activity: Hydrolysis of terminal, non-reducing beta-D- glucosyl residues with release of beta-D-glucose.
Biophysicochemical properties: Kinetic parameters: KM=0.2 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.23 mM for p-nitrophenyl beta-D-fucoside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=3.2 mM for p-nitrophenyl beta-D-galactoside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=1.3 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=1.3 mM for p-nitrophenyl beta-D-xyloside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=1.2 mM for p-nitrophenyl beta-L-arabinoside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.78 mM for p-nitrophenyl beta-D-cellobioside (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=22 mM for cellobiose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.22 mM for cellotriose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.28 mM for cellotetraose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.24 mM for cellopentaose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=0.22 mM for cellohexaose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=2.05 mM for laminaribiose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=1.92 mM for laminaritriose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=13.9 mM for sophorose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588}; KM=38.3 mM for gentiobiose (at pH 5.0) {ECO:0000269|PubMed:14692878, ECO:0000269|PubMed:18308333, ECO:0000269|PubMed:19766588};
Subunit: Homodimer. Formation of the homodimer is zinc-dependent. Dimerization does not increase activity. {ECO:0000269|PubMed:18308333}.
Subcellular location: Secreted {ECO:0000305}.
Similarity: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
Sequence caution: Sequence=AAS07255.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=ABF98424.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=ABF98425.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=ABF98426.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.