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PDBsum entry 2ren
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Hydrolase(acid proteinase)
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PDB id
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2ren
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References listed in PDB file
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Key reference
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Title
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Structure of recombinant human renin, A target for cardiovascular-Active drugs, At 2.5 a resolution.
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Authors
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A.R.Sielecki,
K.Hayakawa,
M.Fujinaga,
M.E.Murphy,
M.Fraser,
A.K.Muir,
C.T.Carilli,
J.A.Lewicki,
J.D.Baxter,
M.N.James.
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Ref.
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Science, 1989,
243,
1346-1351.
[DOI no: ]
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PubMed id
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Abstract
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The x-ray crystal structure of recombinant human renin has been determined.
Molecular dynamics techniques that included crystallographic data as a restraint
were used to improve an initial model based on porcine pepsinogen. The present
agreement factor for data from 8.0 to 2.5 angstroms (A) is 0.236. Some of the
surface loops are poorly determined, and these disordered regions border a 30 A
wide solvent channel. Comparison of renin with other aspartyl proteinases shows
that, although the structural cores and active sites are highly conserved,
surface residues, some of which are critical for specificity, vary greatly (up
to 10A). Knowledge of the actual structure, as opposed to the use of models
based on related enzymes, should facilitate the design of renin inhibitors.
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