UniProt functional annotation for Q9R1M7



	UniProt functional annotation for Q9R1M7

UniProt code: Q9R1M7.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. During the development of neural circuits, plays a role in the synaptic refinement period, restricting spine maturation and growth. By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:25009255, PubMed:24297929). May also play a role in PPP2CB- NMDAR mediated signaling mechanism. {ECO:0000269|PubMed:24297929, ECO:0000269|PubMed:25009255}.

Subunit: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA. Interacts (via C-terminus) with GIT1, but not with GRIA1/GluA1, nor with synaptophysin/SYP; this interaction competes with GIT1 interaction with ARHGEF7/beta-PIX (PubMed:24297929). {ECO:0000269|PubMed:11160393, ECO:0000269|PubMed:11588171, ECO:0000269|PubMed:11929923, ECO:0000269|PubMed:12391275, ECO:0000269|PubMed:24297929}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:11160393, ECO:0000269|PubMed:11588171}; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density. Note=Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.

Tissue specificity: Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord. {ECO:0000269|PubMed:7472412, ECO:0000269|PubMed:7472413, ECO:0000269|PubMed:9891978}.

Developmental stage: Isoform 1 and isoform 2 are expressed in spinal cord, medulla, pons, tegmentum, thalamus and hypothalamus at 15 dpc onwards (PubMed:7472412, PubMed:7472413, PubMed:9891978). In the brain cortex and in the hippocampus, strongly expressed during periods of synapse/spine reorganization at postnatal day 8 (P8) to P15. Expression declines after P25 (at protein level) (PubMed:24297929). {ECO:0000269|PubMed:24297929, ECO:0000269|PubMed:7472412, ECO:0000269|PubMed:7472413, ECO:0000269|PubMed:9891978}.

Ptm: N-glycosylated. {ECO:0000269|PubMed:11160393}.

Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR3A/GRIN3A subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. During the development of neural circuits, plays a role in the synaptic refinement period, restricting spine maturation and growth. By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:25009255, PubMed:24297929). May also play a role in PPP2CB- NMDAR mediated signaling mechanism. {ECO:0000269\|PubMed:24297929, ECO:0000269\|PubMed:25009255}.


Subunit:		Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA. Interacts (via C-terminus) with GIT1, but not with GRIA1/GluA1, nor with synaptophysin/SYP; this interaction competes with GIT1 interaction with ARHGEF7/beta-PIX (PubMed:24297929). {ECO:0000269\|PubMed:11160393, ECO:0000269\|PubMed:11588171, ECO:0000269\|PubMed:11929923, ECO:0000269\|PubMed:12391275, ECO:0000269\|PubMed:24297929}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:11160393, ECO:0000269\|PubMed:11588171}; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density. Note=Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.
Tissue specificity:		Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord. {ECO:0000269\|PubMed:7472412, ECO:0000269\|PubMed:7472413, ECO:0000269\|PubMed:9891978}.
Developmental stage:		Isoform 1 and isoform 2 are expressed in spinal cord, medulla, pons, tegmentum, thalamus and hypothalamus at 15 dpc onwards (PubMed:7472412, PubMed:7472413, PubMed:9891978). In the brain cortex and in the hippocampus, strongly expressed during periods of synapse/spine reorganization at postnatal day 8 (P8) to P15. Expression declines after P25 (at protein level) (PubMed:24297929). {ECO:0000269\|PubMed:24297929, ECO:0000269\|PubMed:7472412, ECO:0000269\|PubMed:7472413, ECO:0000269\|PubMed:9891978}.
Ptm:		N-glycosylated. {ECO:0000269\|PubMed:11160393}.
Similarity:		Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR3A/GRIN3A subfamily. {ECO:0000305}.