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UniProt functional annotation for Q15GI4 | ||
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| UniProt code: Q15GI4. |
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| Organism: | |
Ocimum basilicum (Sweet basil). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Ocimum. |
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| Function: | |
Catalyzes the synthesis of the phenylpropene eugenol from coniferyl acetate (PubMed:16782809, PubMed:17912370). Phenylpropenes are produced by plants as defense compounds with antimicrobial and antianimal properties, or as floral attractants of pollinators (PubMed:16782809). Eugenol is a characteristic aromatic constituent of spices (PubMed:16782809). {ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}. |
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| Catalytic activity: | |
Reaction=acetate + eugenol + NADP(+) = (E)-coniferyl acetate + NADPH; Xref=Rhea:RHEA:24690, ChEBI:CHEBI:4917, ChEBI:CHEBI:30089, ChEBI:CHEBI:47905, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.318; Evidence={ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:24692; Evidence={ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}; |
| Catalytic activity: | |
Reaction=a carboxylate + eugenol + NADP(+) = a coniferyl ester + NADPH; Xref=Rhea:RHEA:32655, ChEBI:CHEBI:4917, ChEBI:CHEBI:29067, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:64292; EC=1.1.1.318; Evidence={ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:32657; Evidence={ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}; |
| Activity regulation: | |
Inhibited by zinc and copper ions (PubMed:16782809). Repressed by 4-bromo-cinnamyl acetate (PubMed:16782809). Desactivated by the competitive inhibitor EMDF ((7S,8S)-ethyl(7,8-methylene)-dihydroferulate) (PubMed:17912370). {ECO:0000269|PubMed:16782809, ECO:0000269|PubMed:17912370}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=5.1 mM for coniferyl acetate (at pH 6.5 and 28 degrees Celsius) {ECO:0000269|PubMed:16782809}; KM=0.57 mM for coniferyl acetate (at pH 6.5 and 25 degrees Celsius) {ECO:0000269|PubMed:17912370}; KM=131 uM for NADPH (at pH 6.5 and 28 degrees Celsius) {ECO:0000269|PubMed:16782809}; Vmax=19.9 nmol/sec/mg enzyme (at pH 6.5 and 28 degrees Celsius) {ECO:0000269|PubMed:16782809}; pH dependence: Optimum pH is 6.5. {ECO:0000269|PubMed:16782809}; |
| Pathway: | |
Aromatic compound metabolism; phenylpropanoid biosynthesis. {ECO:0000269|PubMed:16782809}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:17912370}. |
| Tissue specificity: | |
Trichome glands. {ECO:0000269|PubMed:16782809}. |
| Similarity: | |
Belongs to the NmrA-type oxidoreductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.