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PDBsum entry 2q7n
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Cytokine receptor/cytokine
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PDB id
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2q7n
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Contents |
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* Residue conservation analysis
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PDB id:
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Cytokine receptor/cytokine
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Title:
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Crystal structure of leukemia inhibitory factor in complex with lif receptor (domains 1-5)
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Structure:
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Leukemia inhibitory factor receptor. Chain: a, c. Synonym: lif receptor, lif-r, d-factor/lif receptor, cd118 antigen. Engineered: yes. Leukemia inhibitory factor. Chain: b, d. Synonym: lif, differentiation-stimulating factor, d factor, melanoma- derived lpl inhibitor, mlpli, emfilermin. Engineered: yes
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Gene: lifr. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Homo sapiens. Human. Organism_taxid: 9606.
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Resolution:
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4.00Å
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R-factor:
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0.240
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R-free:
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0.287
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Authors:
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T.Huyton,J.G.Zhang,N.A.Nicola,T.P.J.Garrett
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Key ref:
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T.Huyton
et al.
(2007).
An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor.
Proc Natl Acad Sci U S A,
104,
12737-12742.
PubMed id:
DOI:
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Date:
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07-Jun-07
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Release date:
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31-Jul-07
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PROCHECK
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Headers
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References
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DOI no:
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Proc Natl Acad Sci U S A
104:12737-12742
(2007)
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PubMed id:
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An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor.
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T.Huyton,
J.G.Zhang,
C.S.Luo,
M.Z.Lou,
D.J.Hilton,
N.A.Nicola,
T.P.Garrett.
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ABSTRACT
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Leukemia inhibitory factor (LIF) receptor is a cell surface receptor that
mediates the actions of LIF and other IL-6 type cytokines through the formation
of high-affinity signaling complexes with gp130. Here we present the crystal
structure of a complex of mouse LIF receptor with human LIF at 4.0 A resolution.
The structure is, to date, the largest cytokine receptor fragment determined by
x-ray crystallography. The binding of LIF to its receptor via the central
Ig-like domain is unlike other cytokine receptor complexes that bind ligand
predominantly through their cytokine-binding modules. This structure, in
combination with previous crystallographic studies, also provides a structural
template to understand the formation and orientation of the high-affinity
signaling complex between LIF, LIF receptor, and gp130.
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Selected figure(s)
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Figure 1.
The crystal structure of the hLIF: mLIFR complex. (A) Side
view of the complex illustrating the molecular surface for one
complex, with receptor colored raspberry and ligand colored
orange-gray. The NCS copy of the artificial dimer is shown in
ribbons with receptor colored blue; ligand colored light blue;
disulfide bonds colored orange, and N-linked carbohydrate
colored yellow. (B) A detailed view of the receptor alone with
domains and sites of posttranslational modifications labeled.
Colors are as in A. (C) A domain representation of selected
cytokine receptors illustrating differences in their modular
domain structure.
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Figure 2.
A stereo view of the hLIF:mLIFR binding interface. The
receptor Ig-D4 domain secondary structure is shown as light blue
ribbons. Interface residues are shown as sticks and colored
yellow for receptor, salmon for ligand, and magenta for residues
Pro-51, Lys-153, Phe-156 and Lys-159, previously demonstrated as
essential residues for ligand binding (12).
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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N.Nishishita,
H.Ijiri,
C.Takenaka,
K.Kobayashi,
K.Goto,
E.Kotani,
T.Itoh,
H.Mori,
and
S.Kawamata
(2011).
The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells.
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Biomaterials,
32,
3555-3563.
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S.Le Saux,
F.Rousseau,
F.Barbier,
E.Ravon,
L.Grimaud,
Y.Danger,
J.Froger,
S.Chevalier,
and
H.Gascan
(2010).
Molecular dissection of human interleukin-31-mediated signal transduction through site-directed mutagenesis.
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J Biol Chem,
285,
3470-3477.
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X.Wang,
P.Lupardus,
S.L.Laporte,
and
K.C.Garcia
(2009).
Structural biology of shared cytokine receptors.
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Annu Rev Immunol,
27,
29-60.
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G.Skiniotis,
P.J.Lupardus,
M.Martick,
T.Walz,
and
K.C.Garcia
(2008).
Structural organization of a full-length gp130/LIF-R cytokine receptor transmembrane complex.
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Mol Cell,
31,
737-748.
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PDB code:
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P.J.Lupardus,
and
K.C.Garcia
(2008).
The structure of interleukin-23 reveals the molecular basis of p40 subunit sharing with interleukin-12.
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J Mol Biol,
382,
931-941.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
