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UniProt functional annotation for P29468 | ||
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| UniProt code: P29468. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Polymerase component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation- dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. {ECO:0000269|PubMed:17850751, ECO:0000269|PubMed:18537269}. |
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| Catalytic activity: | |
Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide; Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:11128, Rhea:RHEA-COMP:14647, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83400, ChEBI:CHEBI:140626; EC=2.7.7.19; Evidence={ECO:0000269|PubMed:17850751, ECO:0000269|PubMed:18537269}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17850751}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:17850751}; Note=Binds 2 magnesium ions. Also active with manganese. {ECO:0000269|PubMed:17850751}; |
| Subunit: | |
Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Interacts with FIR1 and RRP6. {ECO:0000269|PubMed:10611239, ECO:0000269|PubMed:10958780, ECO:0000269|PubMed:12819204, ECO:0000269|PubMed:17850751, ECO:0000269|PubMed:18537269, ECO:0000269|PubMed:9236779}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:12819204, ECO:0000269|PubMed:14562095}. |
| Miscellaneous: | |
Present with 17100 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the poly(A) polymerase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.