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PDBsum entry 2q5a

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Isomerase/isomerase inhibitor PDB id
2q5a
Jmol
Contents
Protein chain
145 a.a.
Ligands
PHE-TPO-YCP-NAL-
GLN-NH2
16P
Waters ×167

References listed in PDB file
Key reference
Title Structural basis for high-Affinity peptide inhibition of human pin1.
Authors Y.Zhang, S.Daum, D.Wildemann, X.Z.Zhou, M.A.Verdecia, M.E.Bowman, C.Lücke, T.Hunter, K.P.Lu, G.Fischer, J.P.Noel.
Ref. Acs Chem Biol, 2007, 2, 320-328.
PubMed id 17518432
Abstract
Human Pin1 is a key regulator of cell-cycle progression and plays growth-promoting roles in human cancers. High-affinity inhibitors of Pin1 may provide a unique opportunity for disrupting oncogenic pathways. Here we report two high-resolution X-ray crystal structures of human Pin1 bound to non-natural peptide inhibitors. The structures of the bound high-affinity peptides identify a type-I beta-turn conformation for Pin1 prolyl peptide isomerase domain-peptide binding and an extensive molecular interface for high-affinity recognition. Moreover, these structures suggest chemical elements that may further improve the affinity and pharmacological properties of future peptide-based Pin inhibitors. Finally, an intramolecular hydrogen bond observed in both peptide complexes mimics the cyclic conformation of FK506 and rapamycin. Both FK506 and rapamycin are clinically important inhibitors of other peptidyl-prolyl cis-trans isomerases. This comparative discovery suggests that a cyclic peptide polyketide bridge, like that found in FK506 and rapamycin or a similar linkage, may significantly improve the binding affinity of structure-based Pin1 inhibitors.
PROCHECK
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