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PDBsum entry 2ptt
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protein ligands Protein-protein interface(s) links
Immune system PDB id
2ptt

 

 

 

 

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Contents
Protein chains
103 a.a. *
108 a.a. *
Ligands
SO4
Waters ×213
* Residue conservation analysis
PDB id:
2ptt
Name: Immune system
Title: Structure of nk cell receptor 2b4 (cd244) bound to its ligand cd48
Structure: Cd48 antigen. Chain: a. Fragment: ig-like c2-type1, d1, 2b4-binding domain. Synonym: mrc ox-45 surface antigen, bcm1 surface antigen, blast-1, hm48-1, sgp-60. Engineered: yes. Mutation: yes. Natural killer cell receptor 2b4. Chain: b.
Source: Mus musculus. House mouse. Organism_taxid: 10090. Strain: c57bl/6. Gene: cd48, bcm-1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cd244, 2b4, nmrk.
Resolution:
1.63Å     R-factor:   0.193     R-free:   0.244
Authors: L.Deng,C.A.Velikovsky,R.A.Mariuzza
Key ref: C.A.Velikovsky et al. (2007). Structure of natural killer receptor 2B4 bound to CD48 reveals basis for heterophilic recognition in signaling lymphocyte activation molecule family. Immunity, 27, 572-584. PubMed id: 17950006
Date:
08-May-07     Release date:   20-Nov-07    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P18181  (CD48_MOUSE) -  CD48 antigen from Mus musculus
Seq:
Struc: 		240 a.a.
103 a.a.*
Protein chain
Pfam   ArchSchema ?
Q07763  (CD244_MOUSE) -  Natural killer cell receptor 2B4 from Mus musculus
Seq:
Struc: 		397 a.a.
108 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 

 
Immunity 27:572-584 (2007)
PubMed id: 17950006  
 
 
Structure of natural killer receptor 2B4 bound to CD48 reveals basis for heterophilic recognition in signaling lymphocyte activation molecule family.
C.A.Velikovsky, L.Deng, L.K.Chlewicki, M.M.Fernández, V.Kumar, R.A.Mariuzza.
 
  ABSTRACT  
 
Natural killer (NK) cells eliminate virally infected and tumor cells. Among the receptors regulating NK cell function is 2B4 (CD244), a member of the signaling lymphocyte-activation molecule (SLAM) family that binds CD48. 2B4 is the only heterophilic receptor of the SLAM family, whose other members, e.g., NK-T-B-antigen (NTB-A), are self-ligands. We determined the structure of the complex between the N-terminal domains of mouse 2B4 and CD48, as well as the structures of unbound 2B4 and CD48. The complex displayed an association mode related to, yet distinct from, that of the NTB-A dimer. Binding was accompanied by the rigidification of flexible 2B4 regions containing most of the polymorphic residues across different species and receptor isoforms. We propose a model for 2B4-CD48 interactions that permits the intermixing of SLAM receptors with major histocompatibility complex-specific receptors in the NK cell immune synapse. This analysis revealed the basis for heterophilic recognition within the SLAM family.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22902367 O.J.Harrison, J.Vendome, J.Brasch, X.Jin, S.Hong, P.S.Katsamba, G.Ahlsen, R.B.Troyanovsky, S.M.Troyanovsky, B.Honig, and L.Shapiro (2012).
Nectin ectodomain structures reveal a canonical adhesive interface.
  Nat Struct Mol Biol, 19, 906-915.
PDB codes: 4fmf 4fmk 4fn0 4fom 4fqp 4frw 4fs0
21219180 J.L.Cannons, S.G.Tangye, and P.L.Schwartzberg (2011).
SLAM family receptors and SAP adaptors in immunity.
  Annu Rev Immunol, 29, 665-705.  
21217702 T.Hashiguchi, T.Ose, M.Kubota, N.Maita, J.Kamishikiryo, K.Maenaka, and Y.Yanagi (2011).
Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM.
  Nat Struct Mol Biol, 18, 135-141.
PDB codes: 3alw 3alx 3alz
  20300214 A.Veillette (2010).
SLAM-family receptors: immune regulators with or without SAP-family adaptors.
  Cold Spring Harb Perspect Biol, 2, a002469.  
19818651 J.Back, E.L.Malchiodi, S.Cho, L.Scarpellino, P.Schneider, M.C.Kerzic, R.A.Mariuzza, and W.Held (2009).
Distinct conformations of Ly49 natural killer cell receptors mediate MHC class I recognition in trans and cis.
  Immunity, 31, 598-608.
PDB codes: 3g8k 3g8l
19426233 K.Chattopadhyay, E.Lazar-Molnar, Q.Yan, R.Rubinstein, C.Zhan, V.Vigdorovich, U.A.Ramagopal, J.Bonanno, S.G.Nathenson, and S.C.Almo (2009).
Sequence, structure, function, immunity: structural genomics of costimulation.
  Immunol Rev, 229, 356-386.  
19499526 S.O.Mathew, K.K.Rao, J.R.Kim, N.D.Bambard, and P.A.Mathew (2009).
Functional role of human NK cell receptor 2B4 (CD244) isoforms.
  Eur J Immunol, 39, 1632-1641.  
19648922 Z.Dong, M.E.Cruz-Munoz, M.C.Zhong, R.Chen, S.Latour, and A.Veillette (2009).
Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells.
  Nat Immunol, 10, 973-980.  
  18783178 B.Rubin, J.Riond, L.Courtiade, N.Roullet, and J.E.Gairin (2008).
The innate immune system recognizes and regulates major histocompatibility complex class I (MHCI) expression on MHCIlow tumor cells.
  Cancer Immun, 8, 14.  
18439809 Y.T.Bryceson, and E.O.Long (2008).
Line of attack: NK cell specificity and integration of signals.
  Curr Opin Immunol, 20, 344-352.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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