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PDBsum entry 2opo
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References listed in PDB file
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Key reference
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Title
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Three-Dimensional structure of the cross-Reactive pollen allergen che a 3: visualizing cross-Reactivity on the molecular surfaces of weed, Grass, And tree pollen allergens.
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Authors
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P.Verdino,
R.Barderas,
M.Villalba,
K.Westritschnig,
R.Valenta,
R.Rodriguez,
W.Keller.
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Ref.
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J Immunol, 2008,
180,
2313-2321.
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PubMed id
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Abstract
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Two EF-hand calcium-binding allergens (polcalcins) occur in the pollen of a wide
variety of unrelated plants as highly cross-reactive allergenic molecules. We
report the expression, purification, immunological characterization, and the
1.75-A crystal structure of recombinant Che a 3 (rChe a 3), the polcalcin from
the weed Chenopodium album. The three-dimensional structure of rChe a 3
resembles an alpha-helical fold that is essentially identical with that of the
two EF-hand allergens from birch pollen, Bet v 4, and timothy grass pollen, Phl
p 7. The extensive cross-reactivity between Che a 3 and Phl p 7 is demonstrated
by competition experiments with IgE Abs from allergic patients as well as
specific Ab probes. Amino acid residues that are conserved for the two EF-hand
allergen family were identified in multiple sequence alignments of polcalcins
from 15 different plants. Next, the three-dimensional structures of rChe a 3,
rPhl p 7, and rBet v 4 were used to identify conserved amino acids with high
surface exposition to visualize surface patches as potential targets for the
polyclonal IgE Ab response of allergic patients. The essentially identical
three-dimensional structures of rChe a 3, rPhl p 7, and rBet v 4 explain the
extensive cross-reactivity of allergic patients IgE Abs with two EF-hand
allergens from unrelated plants. In addition, analyzing the three-dimensional
structures of cross-reactive Ags for conserved and surface exposed amino acids
may be a first approach to mapping the conformational epitopes on
disease-related Ags that are recognized by polyclonal patient Abs.
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Secondary reference #1
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Title
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Profilin (che a 2) and polcalcin (che a 3) are relevant allergens of chenopodium album pollen: isolation, Amino acid sequences, And immunologic properties.
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Authors
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R.Barderas,
M.Villalba,
C.Y.Pascual,
E.Batanero,
R.Rodríguez.
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Ref.
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J Allergy Clin Immunol, 2004,
113,
1192-1198.
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PubMed id
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