PDBsum entry 2onj

Hydrolase

PDB id: 2onj

Contents

Protein chains

578 a.a.

Ligands

ANP ×2

Metals

_NA ×4

Waters ×20

References listed in PDB file

Key reference

• Title: Structure of the multidrug abc transporter sav1866 from staphylococcus aureus in complex with AMP-Pnp.
• Authors: R.J.Dawson, K.P.Locher.
• Ref.: Febs Lett, 2007, 581, 935-938.
• PubMed id: 17303126

Abstract

Staphylococcus aureus Sav1866 is a bacterial homolog of the human ABC transporter Mdr1 that causes multidrug resistance in cancer cells. We report the crystal structure of Sav1866 in complex with adenosine-5'-(beta,gamma-imido)triphosphate (AMP-PNP) at 3.4A resolution and compare it with the previously determined structure of Sav1866 with bound ADP. Besides differences in the ATP-binding sites, no significant conformational changes were observed. The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains.