PDBsum entry 2oau

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Membrane protein PDB id
Protein chains
(+ 1 more) 254 a.a.

References listed in PDB file
Key reference
Title Structures of the prokaryotic mechanosensitive channels mscl and mscs
Authors S.Steinbacher, R.Bass, P.Strop, D.C.Rees.
PubMed id -1
No abstract given.
Secondary reference #1
Title Crystal structure of escherichia coli mscs, A voltage-Modulated and mechanosensitive channel.
Authors R.B.Bass, P.Strop, M.Barclay, D.C.Rees.
Ref. Science, 2002, 298, 1582-1587. [DOI no: 10.1126/science.1077945]
PubMed id 12446901
Full text Abstract
Figure 2.
Fig. 2. Ribbon diagrams of MscS. (A) The polypeptide fold of a MscS subunit, viewed perpendicular to the sevenfold axis, with a rainbow gradient coloring scheme from the NH[2]- terminus (blue) to the COOH-terminus (red). The membrane-spanning helices in the transmembrane domain are labeled TM1, TM2, and TM3. The two remaining domains of the monomer, located in the cytoplasm, as well as location of the strands, are also indicated. Brackets denote approximate dimensions of the molecule in both (A) and (B). (B) The MscS heptamer viewed down the sevenfold axis, looking into the permeation pathway from outside the cell. The coloring of the subunits is as in (C). (C) Stereo side view of the MscS heptamer, viewed from the same direction as in (A), with each subunit represented in a separate color. The orientation of this view is such that the periplasm would be at the top, and the cytoplasm would be at the bottom of the figure. This figure was prepared with MOLSCRIPT (52) and RASTER-3D (53).
Figure 4.
Fig. 4. Model for gating the MscS channel. The transmembrane region of the channel, in an open state, is shown in an expanded view, with one subunit highlighted in dark blue and the remaining six subunits represented in transparent light blue. The side chains of arginine residues at positions 46, 74, and 88 are depicted as larger, colored ball and sticks in the highlighted subunit and as smaller, blue ball and sticks in the remaining subunits. The inferred position of the membrane bilayer is schematically indicated as a gray box. Arrows drawn normal and parallel to the plane of the membrane denote the likely directions of force when depolarization and mechanical stress are applied to the membrane, respectively. Curved arrow schematically illustrates the motion the TM1 and TM2 helices could exhibit in response to either or both of these forces.
The above figures are reproduced from the cited reference with permission from the AAAs
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