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PDBsum entry 2o39
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Viral protein/immune system
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PDB id
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2o39
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References listed in PDB file
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Key reference
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Title
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Adenovirus type 11 binding alters the conformation of its receptor cd46.
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Authors
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B.D.Persson,
D.M.Reiter,
M.Marttila,
Y.F.Mei,
J.M.Casasnovas,
N.Arnberg,
T.Stehle.
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Ref.
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Nat Struct Mol Biol, 2007,
14,
164-166.
[DOI no: ]
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PubMed id
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Abstract
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Adenoviruses (Ads) are important human pathogens and valuable gene delivery
vehicles. We report here the crystal structure of the species B Ad11 knob
complexed with the Ad11-binding region of its receptor CD46. The conformation of
bound CD46 differs profoundly from its unbound state, with the bent surface
structure straightened into an elongated rod. This mechanism of interaction is
likely to be conserved among many pathogens that target CD46 or related
molecules.
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Figure 1.
Figure 1. Structure of the Ad11 knob complexed with CD46
SCR1-SCR2. (a) Ribbon drawing of the complex. The three
subunits forming the Ad11 knob are blue, green and gray; bound
CD46 SCR1-SCR2 is red. Glycosylated residues Asn49 and Asn80 are
red. The glycan attached to Asn80 is yellow. (b) Surface
representation of a CD46 SCR1-SCR2 molecule bound to two Ad11
knob protomers, colored as in a. CD46 has been moved away from
the two Ad11 knob protomers by about 5 Å to highlight
shape complementarity. Views in a and b are from opposite
directions. (c) 2F[o] – F[c] electron density map, contoured
at 1.0  and
showing interactions between CD46 and the Ad11 knob.
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Figure 2.
Figure 2. Interaction between the Ad11 knob and CD46 SCR1-SCR2.
(a) Ribbon tracing of the complex, colored as in Figure 1.
(b–d) The three major contact regions (A–C) of Ad11 knob.
Nitrogen and oxygen atoms are blue and red, respectively.
Hydrogen bonds and salt bridges are represented with dashed
lines. (e) Superimposition of SCR2 domains of unliganded^13
(blue) and liganded CD46 (red). The two Ad11 knob protomers
interacting with CD46 are shown in gray. Arrow indicates the
rearrangement of SCR1 upon binding to Ad11 knob. (f) Close-up
view of the interaction. Key residues of CD46 that interact with
Ad11 knob are shown in orange. (g) Structure of unliganded CD46.
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The above figures are
reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2007,
14,
164-166)
copyright 2007.
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