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PDBsum entry 2o39
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protein ligands metals Protein-protein interface(s) links
Viral protein/immune system PDB id
2o39

 

 

 

 

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Contents
Protein chains
197 a.a. *
126 a.a. *
Ligands
NAG-NAG-BMA ×2
Metals
_CA ×3
Waters ×34
* Residue conservation analysis
PDB id:
2o39
Name: Viral protein/immune system
Title: Human adenovirus type 11 knob in complex with domains scr1 and scr2 of cd46 (membrane cofactor protein, mcp)
Structure: Fiber protein. Chain: a, b. Fragment: residues 129-325. Synonym: piv. Engineered: yes. Membrane cofactor protein. Chain: c, d. Fragment: scr1 and scr 2 domains. Synonym: trophoblast leukocyte common antigen, tlx, cd46 antigen.
Source: Human adenovirus 11p. Organism_taxid: 343462. Strain: slobiski. Gene: piv. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.
Resolution:
2.85Å     R-factor:   0.238     R-free:   0.273
Authors: D.B.Persson,D.M.Reiter,N.Arnberg,T.Stehle
Key ref:
B.D.Persson et al. (2007). Adenovirus type 11 binding alters the conformation of its receptor CD46. Nat Struct Mol Biol, 14, 164-166. PubMed id: 17220899 DOI: 10.1038/nsmb1190
Date:
01-Dec-06     Release date:   09-Jan-07    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P35774  (SPIKE_ADE1P) -  Fiber protein from Human adenovirus B serotype 11 (strain Slobiski)
Seq:
Struc: 		325 a.a.
197 a.a.
Protein chains
Pfam   ArchSchema ?
P15529  (MCP_HUMAN) -  Membrane cofactor protein from Homo sapiens
Seq:
Struc: 		392 a.a.
126 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1038/nsmb1190 Nat Struct Mol Biol 14:164-166 (2007)
PubMed id: 17220899  
 
 
Adenovirus type 11 binding alters the conformation of its receptor CD46.
B.D.Persson, D.M.Reiter, M.Marttila, Y.F.Mei, J.M.Casasnovas, N.Arnberg, T.Stehle.
 
  ABSTRACT  
 
Adenoviruses (Ads) are important human pathogens and valuable gene delivery vehicles. We report here the crystal structure of the species B Ad11 knob complexed with the Ad11-binding region of its receptor CD46. The conformation of bound CD46 differs profoundly from its unbound state, with the bent surface structure straightened into an elongated rod. This mechanism of interaction is likely to be conserved among many pathogens that target CD46 or related molecules.
 
  Selected figure(s)  
 
Figure 1.
Figure 1. Structure of the Ad11 knob complexed with CD46 SCR1-SCR2. (a) Ribbon drawing of the complex. The three subunits forming the Ad11 knob are blue, green and gray; bound CD46 SCR1-SCR2 is red. Glycosylated residues Asn49 and Asn80 are red. The glycan attached to Asn80 is yellow. (b) Surface representation of a CD46 SCR1-SCR2 molecule bound to two Ad11 knob protomers, colored as in a. CD46 has been moved away from the two Ad11 knob protomers by about 5 Å to highlight shape complementarity. Views in a and b are from opposite directions. (c) 2F[o] – F[c] electron density map, contoured at 1.0 and showing interactions between CD46 and the Ad11 knob. 		Figure 2.
Figure 2. Interaction between the Ad11 knob and CD46 SCR1-SCR2. (a) Ribbon tracing of the complex, colored as in Figure 1. (b–d) The three major contact regions (A–C) of Ad11 knob. Nitrogen and oxygen atoms are blue and red, respectively. Hydrogen bonds and salt bridges are represented with dashed lines. (e) Superimposition of SCR2 domains of unliganded^13 (blue) and liganded CD46 (red). The two Ad11 knob protomers interacting with CD46 are shown in gray. Arrow indicates the rearrangement of SCR1 upon binding to Ad11 knob. (f) Close-up view of the interaction. Key residues of CD46 that interact with Ad11 knob are shown in orange. (g) Structure of unliganded CD46.
 
  The above figures are reprinted by permission from Macmillan Publishers Ltd: Nat Struct Mol Biol (2007, 14, 164-166) copyright 2007.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21366418 D.Henaff, S.Salinas, and E.J.Kremer (2011).
An adenovirus traffic update: from receptor engagement to the nuclear pore.
  Future Microbiol, 6, 179-192.  
21151139 E.C.Nilsson, R.J.Storm, J.Bauer, S.M.Johansson, A.Lookene, J.Ångström, M.Hedenström, T.L.Eriksson, L.Frängsmyr, S.Rinaldi, H.J.Willison, F.Pedrosa Domellöf, T.Stehle, and N.Arnberg (2011).
The GD1a glycan is a cellular receptor for adenoviruses causing epidemic keratoconjunctivitis.
  Nat Med, 17, 105-109.
PDB code: 3n0i
20941397 B.D.Persson, N.B.Schmitz, C.Santiago, G.Zocher, M.Larvie, U.Scheu, J.M.Casasnovas, and T.Stehle (2010).
Structure of the extracellular portion of CD46 provides insights into its interactions with complement proteins and pathogens.
  PLoS Pathog, 6, 0.
PDB code: 3o8e
  20057080 C.Santiago, A.Gutiérrez-Rodríguez, P.A.Tucker, T.Stehle, and J.M.Casasnovas (2010).
Crystallization and preliminary crystallographic analysis of the measles virus hemagglutinin in complex with the CD46 receptor.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 91-94.  
20010840 C.Santiago, M.L.Celma, T.Stehle, and J.M.Casasnovas (2010).
Structure of the measles virus hemagglutinin bound to the CD46 receptor.
  Nat Struct Mol Biol, 17, 124-129.
PDB code: 3inb
  20376613 J.G.Smith, C.M.Wiethoff, P.L.Stewart, and G.R.Nemerow (2010).
Adenovirus.
  Curr Top Microbiol Immunol, 343, 195-224.  
  19788385 J.S.Senac, K.Doronin, S.J.Russell, D.F.Jelinek, P.R.Greipp, and M.A.Barry (2010).
Infection and killing of multiple myeloma by adenoviruses.
  Hum Gene Ther, 21, 179-190.  
20071571 K.Cupelli, S.Müller, B.D.Persson, M.Jost, N.Arnberg, and T.Stehle (2010).
Structure of adenovirus type 21 knob in complex with CD46 reveals key differences in receptor contacts among species B adenoviruses.
  J Virol, 84, 3189-3200.
PDB codes: 3l88 3l89
19647886 A.Sharma, X.Li, D.S.Bangari, and S.K.Mittal (2009).
Adenovirus receptors and their implications in gene delivery.
  Virus Res, 143, 184-194.  
18987134 B.D.Persson, S.Müller, D.M.Reiter, B.B.Schmitt, M.Marttila, C.V.Sumowski, S.Schweizer, U.Scheu, C.Ochsenfeld, N.Arnberg, and T.Stehle (2009).
An arginine switch in the species B adenovirus knob determines high-affinity engagement of cellular receptor CD46.
  J Virol, 83, 673-686.
PDB codes: 3exv 3exw 3f0y
19019405 G.R.Nemerow, L.Pache, V.Reddy, and P.L.Stewart (2009).
Insights into adenovirus host cell interactions from structural studies.
  Virology, 384, 380-388.  
19516278 H.Matsui, F.Sakurai, S.Kurachi, K.Tashiro, K.Sugio, K.Kawabata, K.Yamanishi, and H.Mizuguchi (2009).
Development of fiber-substituted adenovirus vectors containing foreign peptides in the adenovirus serotype 35 fiber knob.
  Gene Ther, 16, 1050-1057.  
19307010 H.Wang, S.Tuve, D.D.Erdman, and A.Lieber (2009).
Receptor usage of a newly emergent adenovirus type 14.
  Virology, 387, 436-441.  
19199789 L.Sandberg, P.Papareddy, J.Silver, A.Bergh, and Y.F.Mei (2009).
Replication-competent Ad11p vector (RCAd11p) efficiently transduces and replicates in hormone-refractory metastatic prostate cancer cells.
  Hum Gene Ther, 20, 361-373.  
19342221 T.Stehle, and J.M.Casasnovas (2009).
Specificity switching in virus-receptor complexes.
  Curr Opin Struct Biol, 19, 181-188.  
19088268 W.C.Russell (2009).
Adenoviruses: update on structure and function.
  J Gen Virol, 90, 1.  
18323776 B.Amstutz, M.Gastaldelli, S.Kälin, N.Imelli, K.Boucke, E.Wandeler, J.Mercer, S.Hemmi, and U.F.Greber (2008).
Subversion of CtBP1-controlled macropinocytosis by human adenovirus serotype 3.
  EMBO J, 27, 956-969.  
17914026 C.J.Fang, V.Fremeaux-Bacchi, M.K.Liszewski, G.Pianetti, M.Noris, T.H.Goodship, and J.P.Atkinson (2008).
Membrane cofactor protein mutations in atypical hemolytic uremic syndrome (aHUS), fatal Stx-HUS, C3 glomerulonephritis, and the HELLP syndrome.
  Blood, 111, 624-632.  
18753195 H.Wang, Y.Liu, Z.Li, S.Tuve, D.Stone, O.Kalyushniy, D.Shayakhmetov, C.L.Verlinde, T.Stehle, J.McVey, A.Baker, K.W.Peng, S.Roffler, and A.Lieber (2008).
In vitro and in vivo properties of adenovirus vectors with increased affinity to CD46.
  J Virol, 82, 10567-10579.  
18524830 L.Pache, S.Venkataraman, V.S.Reddy, and G.R.Nemerow (2008).
Structural variations in species B adenovirus fibers impact CD46 association.
  J Virol, 82, 7923-7931.
PDB code: 3cnc
18974862 S.Tuve, H.Wang, J.D.Jacobs, R.C.Yumul, D.F.Smith, and A.Lieber (2008).
Role of cellular heparan sulfate proteoglycans in infection of human adenovirus serotype 3 and 35.
  PLoS Pathog, 4, e1000189.  
17898059 H.Wang, Y.C.Liaw, D.Stone, O.Kalyuzhniy, I.Amiraslanov, S.Tuve, C.L.Verlinde, D.Shayakhmetov, T.Stehle, S.Roffler, and A.Lieber (2007).
Identification of CD46 binding sites within the adenovirus serotype 35 fiber knob.
  J Virol, 81, 12785-12792.
PDB code: 2qlk
17805302 S.Murakami, F.Sakurai, K.Kawabata, N.Okada, T.Fujita, A.Yamamoto, T.Hayakawa, and H.Mizuguchi (2007).
Interaction of penton base Arg-Gly-Asp motifs with integrins is crucial for adenovirus serotype 35 vector transduction in human hematopoietic cells.
  Gene Ther, 14, 1525-1533.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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