PDBsum entry 2o25

Ligase

PDB id: 2o25

Contents

Protein chains

199 a.a. *

157 a.a. *

Waters ×39

* Residue conservation analysis

PDB id:

2o25

Name:

Ligase

Title:

Ubiquitin-conjugating enzyme e2-25 kda complexed with sumo-1- conjugating enzyme ubc9

Structure:

Ubiquitin-conjugating enzyme e2-25 kda. Chain: a, b. Synonym: ubiquitin-protein ligase, ubiquitin carrier protein, e225k, huntingtin-interacting protein 2, hip-2. Engineered: yes. Sumo-1-conjugating enzyme ubc9. Chain: c, d. Synonym: sumo-1-protein ligase, ubiquitin-conjugating enzyme e2 i, ubiquitin-protein ligase i, ubiquitin carrier protein i, ubiquitin

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hip2, lig. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: ube2i, ubc9, ubce9.

Resolution:

2.60Å R-factor: 0.231 R-free: 0.291

Authors:

J.R.Walker,G.V.Avvakumov,S.Xue,E.M.Newman,F.Mackenzie,J.Weigelt, M.Sundstrom,C.H.Arrowsmith,A.M.Edwards,A.Bochkarev,S.Dhe-Paganon, Structural Genomics Consortium (Sgc)

Key ref:

J.R.Walker et al. A novel and unexpected complex between the sumo-1-Conjugating enzyme ubc9 and the ubiquitin-Conjugating enzyme e2-25 kda. To be published, .

Date:

29-Nov-06 Release date: 16-Jan-07

Protein chains

P61086  (UBE2K_HUMAN) -  Ubiquitin-conjugating enzyme E2 K from Homo sapiens

Seq: 200 a.a.

Struc: 199 a.a.

Protein chains

P63279  (UBC9_HUMAN) -  SUMO-conjugating enzyme UBC9 from Homo sapiens

Seq: 158 a.a.

Struc: 157 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine