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PDBsum entry 2nvz
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Transcription,transferase/DNA-RNA hybrid
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PDB id
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2nvz
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Contents |
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1398 a.a.
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1096 a.a.
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266 a.a.
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193 a.a.
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83 a.a.
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133 a.a.
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119 a.a.
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65 a.a.
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114 a.a.
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46 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis.
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Authors
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D.Wang,
D.A.Bushnell,
K.D.Westover,
C.D.Kaplan,
R.D.Kornberg.
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Ref.
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Cell, 2006,
127,
941-954.
[DOI no: ]
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PubMed id
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Abstract
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New structures of RNA polymerase II (pol II) transcribing complexes reveal a
likely key to transcription. The trigger loop swings beneath a correct
nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the
active center and forming an extensive network of interactions with the NTP
base, sugar, phosphates, and additional pol II residues. A histidine side chain
in the trigger loop, precisely positioned by these interactions, may literally
"trigger" phosphodiester bond formation. Recognition and catalysis are
thus coupled, ensuring the fidelity of transcription.
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Figure 1.
Figure 1. A and E Sites in the Pol II Transcribing Complex
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Figure 5.
Figure 5. Proposed Role of His1085 in Phosphodiester Bond
Formation
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The above figures are
reprinted
by permission from Cell Press:
Cell
(2006,
127,
941-954)
copyright 2006.
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