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PDBsum entry 2nvz

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Transcription,transferase/DNA-RNA hybrid PDB id
2nvz
Jmol
Contents
Protein chains
1398 a.a.
1096 a.a.
266 a.a.
193 a.a.
83 a.a.
133 a.a.
119 a.a.
65 a.a.
114 a.a.
46 a.a.
DNA/RNA
Ligands
UTP
Metals
_ZN ×8
_MG ×2

References listed in PDB file
Key reference
Title Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis.
Authors D.Wang, D.A.Bushnell, K.D.Westover, C.D.Kaplan, R.D.Kornberg.
Ref. Cell, 2006, 127, 941-954. [DOI no: 10.1016/j.cell.2006.11.023]
PubMed id 17129781
Abstract
New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.
Figure 1.
Figure 1. A and E Sites in the Pol II Transcribing Complex
Figure 5.
Figure 5. Proposed Role of His1085 in Phosphodiester Bond Formation
The above figures are reprinted by permission from Cell Press: Cell (2006, 127, 941-954) copyright 2006.
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 Headers