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PDBsum entry 2nn2
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Transcription
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PDB id
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2nn2
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Contents |
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* Residue conservation analysis
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PDB id:
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Transcription
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Title:
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Crystal structure of the btb domain from the lrf/zbtb7 transcriptional regulator
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Structure:
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Zinc finger and btb domain-containing protein 7a. Chain: a, b. Fragment: btb/poz domain, residues 1-131. Synonym: leukemia/lymphoma- related factor, factor that binds to inducer of short transcripts protein 1, factor binding ist protein 1, fbi-1, HIV-1 1st-binding protein 1, ttf-i-interacting peptide 21, tip21. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: zbtb7a, fbi1, lrf, zbtb7. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Biol. unit:
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Dimer (from
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Resolution:
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2.10Å
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R-factor:
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0.216
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R-free:
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0.267
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Authors:
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P.J.Stogios,L.Chen,G.G.Prive
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Key ref:
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P.J.Stogios
et al.
(2007).
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.
Protein Sci,
16,
336-342.
PubMed id:
DOI:
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Date:
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23-Oct-06
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Release date:
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21-Nov-06
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PROCHECK
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Headers
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References
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O95365
(ZBT7A_HUMAN) -
Zinc finger and BTB domain-containing protein 7A from Homo sapiens
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Seq:
Struc:
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584 a.a.
117 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Protein Sci
16:336-342
(2007)
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PubMed id:
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Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.
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P.J.Stogios,
L.Chen,
G.G.Privé.
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ABSTRACT
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BTB-zinc finger (BTB-ZF) proteins are transcription regulators with roles in
development, differentiation, and oncogenesis. In these proteins, the BTB domain
(also known as the POZ domain) is a protein-protein interaction motif that
contains a dimerization interface, a possible oligomerization surface, and
surfaces for interactions with other factors, including nuclear co-repressors
and histone deacetylases. The BTB-ZF protein LRF (also known as ZBTB7, FBI-1,
OCZF, and Pokemon) is a master regulator of oncogenesis, and represses the
transcription of a variety of important genes, including the ARF, c-fos, and
c-myc oncogenes and extracellular matrix genes. We determined the crystal
structure of the BTB domain from human LRF to 2.1 A and observed the canonical
BTB homodimer fold. However, novel features are apparent on the surface of the
homodimer, including differences in the lateral groove and charged pocket
regions. The residues that line the lateral groove have little similarity with
the equivalent residues from the BCL6 BTB domain, and we show that the
17-residue BCL6 Binding Domain (BBD) from the SMRT co-repressor does not bind to
the LRF BTB domain.
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Selected figure(s)
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The above figure is
reprinted
by permission from the Protein Society:
Protein Sci
(2007,
16,
336-342)
copyright 2007.
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Figure was
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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L.C.Cerchietti,
A.F.Ghetu,
X.Zhu,
G.F.Da Silva,
S.Zhong,
M.Matthews,
K.L.Bunting,
J.M.Polo,
C.Farès,
C.H.Arrowsmith,
S.N.Yang,
M.Garcia,
A.Coop,
A.D.Mackerell,
G.G.Privé,
and
A.Melnick
(2010).
A small-molecule inhibitor of BCL6 kills DLBCL cells in vitro and in vivo.
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Cancer Cell,
17,
400-411.
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PDB code:
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T.Ogura,
K.I.Tong,
K.Mio,
Y.Maruyama,
H.Kurokawa,
C.Sato,
and
M.Yamamoto
(2010).
Keap1 is a forked-stem dimer structure with two large spheres enclosing the intervening, double glycine repeat, and C-terminal domains.
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Proc Natl Acad Sci U S A,
107,
2842-2847.
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N.Ito,
M.Watanabe-Matsui,
K.Igarashi,
and
K.Murayama
(2009).
Crystal structure of the Bach1 BTB domain and its regulation of homodimerization.
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Genes Cells,
14,
167-178.
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G.W.Buchko,
O.Litvinova,
H.Robinson,
A.F.Yakunin,
and
M.A.Kennedy
(2008).
Functional and structural characterization of DR_0079 from Deinococcus radiodurans, a novel Nudix hydrolase with a preference for cytosine (deoxy)ribonucleoside 5'-Di- and triphosphates.
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Biochemistry,
47,
6571-6582.
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PDB code:
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M.A.Stead,
G.O.Rosbrook,
J.M.Hadden,
C.H.Trinh,
S.B.Carr,
and
S.C.Wright
(2008).
Structure of the wild-type human BCL6 POZ domain.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
64,
1101-1104.
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PDB code:
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M.Laudes,
R.Bilkovski,
F.Oberhauser,
A.Droste,
M.Gomolka,
U.Leeser,
M.Udelhoven,
and
W.Krone
(2008).
Transcription factor FBI-1 acts as a dual regulator in adipogenesis by coordinated regulation of cyclin-A and E2F-4.
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J Mol Med,
86,
597-608.
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S.Parekh,
G.Privé,
and
A.Melnick
(2008).
Therapeutic targeting of the BCL6 oncogene for diffuse large B-cell lymphomas.
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Leuk Lymphoma,
49,
874-882.
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K.Apostolopoulou,
I.S.Pateras,
K.Evangelou,
P.K.Tsantoulis,
M.Liontos,
C.Kittas,
D.G.Tiniakos,
A.Kotsinas,
C.Cordon-Cardo,
and
V.G.Gorgoulis
(2007).
Gene amplification is a relatively frequent event leading to ZBTB7A (Pokemon) overexpression in non-small cell lung cancer.
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J Pathol,
213,
294-302.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
