PDBsum entry 2nb4

Unknown function

PDB id: 2nb4

Contents

Protein chain

105 a.a.

PDB id:

2nb4

Name:

Unknown function

Title:

Solution structure of q388a3 pdz domain

Structure:

Putative uncharacterized protein. Chain: a. Fragment: unp residues 1528-1630. Engineered: yes

Source:

Trypanosoma brucei brucei treu927. Organism_taxid: 185431. Strain: 927/4 gutat10.1. Gene: tb10.61.1430. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

S.Mei

Key ref:

S.Mei et al. (2016). Solution structure of Q388A3 PDZ domain from Trypanosoma brucei. J Struct Biol, 194, 214-217. PubMed id: 26917351 DOI: 10.1016/j.jsb.2016.02.018

Date:

24-Jan-16 Release date: 24-Feb-16

Protein chain

Q388A3  (Q388A3_TRYB2) -  Uncharacterized protein from Trypanosoma brucei brucei (strain 927/4 GUTat10.1)

Seq: 1634 a.a.

Struc: 105 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.jsb.2016.02.018

J Struct Biol 194:214-217 (2016)

PubMed id: 26917351

Solution structure of Q388A3 PDZ domain from Trypanosoma brucei.

S.Mei, Y.Dong, J.Zhang, X.Zhang, X.Tu.

ABSTRACT

PDZ domains are abundant protein interaction modules that often recognize short amino acid motifs at the C-termini of target proteins and regulate multiple biological processes. So far, no PDZ domain in Trypanosoma brucei, an eukaryotic parasite causing sleeping sickness, has been studied. Q388A3, conserved in the related kinetoplastid parasites, is a 1634-residue protein containing a PDZ domain at its C-terminus. In this work, the solution structure of Q388A3 PDZ domain was solved by NMR spectroscopy. Q388A3 PDZ domain adopts a PDZ-like fold composed by a five-stranded β-sheet capped by two α-helices, which is similar to the PDZ domains from HtrA family proteins. Meanwhile, Q388A3 PDZ domain shows some structural features quite different from HtrA PDZ domain.