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PDBsum entry 2n74
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Viral protein
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PDB id
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2n74
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References listed in PDB file
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Key reference
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Title
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Structural basis for a novel interaction between the ns1 protein derived from the 1918 influenza virus and rig-I.
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Authors
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A.S.Jureka,
A.B.Kleinpeter,
G.Cornilescu,
C.C.Cornilescu,
C.M.Petit.
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Ref.
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Structure, 2015,
23,
2001-2010.
[DOI no: ]
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PubMed id
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Abstract
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The influenza non-structural protein 1 (NS1) plays a critical role in
antagonizing the innate immune response to infection. One interaction that
facilitates this function is between NS1 and RIG-I, one of the main sensors of
influenza virus infection. While NS1 and RIG-I are known to interact, it is
currently unclear whether this interaction is direct or if it is mediated by
other biomolecules. Here we demonstrate a direct, strain-dependent interaction
between the NS1 RNA binding domain (NS1(RBD)) of the influenza A/Brevig
Mission/1918 H1N1 (1918(H1N1)) virus and the second caspase activation and
recruitment domain of RIG-I. Solving the solution structure of the 1918(H1N1)
NS1(RBD) revealed features in a functionally novel region that may facilitate
the observed interaction. The biophysical and structural data herein suggest a
possible mechanism by which strain-specific differences in NS1 modulate
influenza virulence.
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