PDBsum entry 2n3u

Protein binding

PDB id: 2n3u

Contents

Protein chains

131 a.a.

76 a.a.

PDB id:

2n3u

Name:

Protein binding

Title:

Solution structure of the rpn1 t1 site engaging two monoubiquitin molecules

Structure:

26s proteasome regulatory subunit rpn1. Chain: a. Fragment: unp residues 482-612. Synonym: hmg-coa reductase degradation protein 2, proteasome non- atpase subunit 1. Engineered: yes. Ubiquitin-60s ribosomal protein l40. Chain: b, c. Fragment: unp residues 1-76.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: rpn1, hrd2, nas1, rpd1, yhr027c. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human.

NMR struc:

10 models

Authors:

X.Chen,K.J.Walters

Key ref:

Y.Shi et al. (2016). Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Science, 351, . PubMed id: 26912900 DOI: 10.1126/science.aad9421

Date:

10-Jun-15 Release date: 24-Feb-16

Protein chain

P38764  (RPN1_YEAST) -  26S proteasome regulatory subunit RPN1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 993 a.a.

Struc: 131 a.a.

Protein chains

P62987  (RL40_HUMAN) -  Ubiquitin-ribosomal protein eL40 fusion protein from Homo sapiens

Seq: 128 a.a.

Struc: 76 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.?

DOI no: 10.1126/science.aad9421

Science 351: (2016)

PubMed id: 26912900

Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.

Y.Shi, X.Chen, S.Elsasser, B.B.Stocks, G.Tian, B.H.Lee, Y.Shi, N.Zhang, S.A.de Poot, F.Tuebing, S.Sun, J.Vannoy, S.G.Tarasov, J.R.Engen, D.Finley, K.J.Walters.

ABSTRACT

No abstract given.