PDBsum entry 2n25

DNA binding protein

PDB id: 2n25

Contents

Protein chain

29 a.a.

Metals

_ZN

PDB id:

2n25

Name:

DNA binding protein

Title:

Solution structure of miz-1 zinc finger 2

Structure:

Zinc finger and btb domain-containing protein 17. Chain: a. Fragment: unp residues 304-414. Synonym: myc-interacting zinc finger protein 1, miz-1, zinc finger protein 151, zinc finger protein 60. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: miz1, zbtb17, znf151, znf60. Expressed in: escherichia coli. Expression_system_taxid: 469008.

NMR struc:

20 models

Authors:

M.Bedard,P.Lavigne

Key ref:

C.Tremblay et al. (2016). Solution structure of the 13th C2H2 Zinc Finger of Miz-1. Biochem Biophys Res Commun, 473, 471-475. PubMed id: 26972249 DOI: 10.1016/j.bbrc.2016.03.034

Date:

28-Apr-15 Release date: 17-Jun-15

Protein chain

Q13105  (ZBT17_HUMAN) -  Zinc finger and BTB domain-containing protein 17 from Homo sapiens

Seq: 803 a.a.

Struc: 29 a.a.

DOI no: 10.1016/j.bbrc.2016.03.034

Biochem Biophys Res Commun 473:471-475 (2016)

PubMed id: 26972249

Solution structure of the 13th C2H2 Zinc Finger of Miz-1.

C.Tremblay, M.Bédard, M.A.Bonin, P.Lavigne.

ABSTRACT

Miz-1 is a BTB/POZ transcription factor that contains 13C2H2 Zinc Finger domains (ZF). Miz-1 transactivates and represses the transcription of a myriad of genes involved in many aspects of the biology of the cell. The detailed molecular interactions through which Miz-1 controls transcription, including its specific DNA binding via its ZF domains, remain to be understood and documented. In our effort to shed light into the structural biology of Miz-1, we have undertaken the determination of the structure of all its ZF and the characterization of their interactions with cognate DNA. The structure of ZF 1 to 10 have already been solved and characterized. Here, we present the structure of the synthetic Miz-1 ZF13 determined by 2D (1)H-(1)H NMR.