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PDBsum entry 2msb
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a c-Type mannose-Binding protein complexed with an oligosaccharide.
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Authors
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W.I.Weis,
K.Drickamer,
W.A.Hendrickson.
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Ref.
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Nature, 1992,
360,
127-134.
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PubMed id
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Abstract
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C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins
mediate many cell-surface carbohydrate-recognition events. The crystal structure
at 1.7 A resolution of the carbohydrate-recognition domain of rat
mannose-binding protein complexed with an oligomannose
asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the
carbohydrate ligand. Carbohydrate specificity is determined by a network of
coordination and hydrogen bonds that stabilizes the ternary complex of protein,
Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring
carbohydrate-recognition domains in the crystal, enabling multivalent binding to
a single oligosaccharide chain to be visualized directly.
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Secondary reference #1
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Title
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Physical characterization and crystallization of the carbohydrate-Recognition domain of a mannose-Binding protein from rat.
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Authors
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W.I.Weis,
G.V.Crichlow,
H.M.Murthy,
W.A.Hendrickson,
K.Drickamer.
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Ref.
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J Biol Chem, 1991,
266,
20678-20686.
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PubMed id
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Secondary reference #2
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Title
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Structure of the calcium-Dependent lectin domain from a rat mannose-Binding protein determined by mad phasing.
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Authors
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W.I.Weis,
R.Kahn,
R.Fourme,
K.Drickamer,
W.A.Hendrickson.
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Ref.
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Science, 1991,
254,
1608-1615.
[DOI no: ]
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PubMed id
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