PDBsum entry 2ma6

Ligase

PDB id: 2ma6

Contents

Protein chain

61 a.a.

Metals

_ZN ×2

PDB id:

2ma6

Name:

Ligase

Title:

Solution nmr structure of the ring finger domain from the kip1 ubiquitination-promoting e3 complex protein 1 (kpc1/rnf123) from homo sapiens, northeast structural genomics consortium (nesg) target hr8700a

Structure:

E3 ubiquitin-protein ligase rnf123. Chain: a. Fragment: unp residues 1247-1304. Synonym: kip1 ubiquitination-promoting complex protein 1, ring finger protein 123. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fp1477, kpc1, rnf123. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: plasmid

NMR struc:

20 models

Authors:

T.A.Ramelot,Y.Yang,H.Janjua,E.Kohan,H.Wang,R.Xiao,T.B.Acton, J.K.Everett,G.T.Montelione,M.A.Kennedy,Northeast Structural Genomics Consortium (Nesg)

Key ref:

T.A.Ramelot et al. Solution nmr structure of the ring finger domain from kip1 ubiquitination-Promoting e3 complex protein 1 (kpc1/rnf123) from homo sapiens, Northeast structural genomics consortium (nesg) target hr8700a. To be published, .

Date:

28-Jun-13 Release date: 31-Jul-13

Protein chain

Q5XPI4  (RN123_HUMAN) -  E3 ubiquitin-protein ligase RNF123 from Homo sapiens

Seq: 1314 a.a.

Struc: 61 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine