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PDBsum entry 2m7q
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Metal binding protein
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PDB id
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2m7q
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References listed in PDB file
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Key reference
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Title
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The structure of tax1bp1 ubz1+2 provides insight into target specificity and adaptability.
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Authors
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M.A.Ceregido,
M.Spínola amilibia,
L.Buts,
J.Rivera-Torres,
A.Garcia-Pino,
J.Bravo,
N.A.Van nuland.
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Ref.
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J Mol Biol, 2014,
426,
674-690.
[DOI no: ]
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PubMed id
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Abstract
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TAX1BP1 is a novel ubiquitin-binding adaptor protein involved in the negative
regulation of the NF-kappaB transcription factor, which is a key player in
inflammatory responses, immunity and tumorigenesis. TAX1BP1 recruits A20 to the
ubiquitinated signaling proteins TRAF6 and RIP1, leading to their A20-mediated
deubiquitination and the disruption of IL-1-induced and TNF-induced NF-kappaB
signaling, respectively. The two zinc fingers localized at its C-terminus
function as novel ubiquitin-binding domains (UBZ, ubiquitin-binding zinc
finger). Here we present for the first time both the solution and crystal
structures of two classical UBZ domains in tandem within the human TAX1BP1. The
relative orientation of the two domains is slightly different in the X-ray
structure with respect to the NMR structure, indicating some degree of
conformational flexibility, which is rationalized by NMR relaxation data. The
observed degree of flexibility and stability between the two UBZ domains might
have consequences on the recognition mechanism of interacting partners.
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