PDBsum entry 2m3s

Metal binding protein

PDB id: 2m3s

Contents

Protein chain

151 a.a.

Metals

_CA ×4

PDB id:

2m3s

Name:

Metal binding protein

Title:

Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

Structure:

Calmodulin. Chain: a. Fragment: unp residues 1-149. Synonym: cam. Engineered: yes. Mutation: yes

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Gene: calm, cam, rcjmb04_24e7. Expressed in: escherichia coli. Expression_system_taxid: 469008.

NMR struc:

20 models

Authors:

Y.S.Moroz,Y.Wu,H.Cheng,H.Roder,I.V.Korendovych

Key ref:

O.V.Moroz et al. (2013). A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction. Angew Chem Int Ed Engl, 52, 6246-6249. PubMed id: 23630096 DOI: 10.1002/anie.201302339

Date:

25-Jan-13 Release date: 24-Jul-13

Protein chain

P62149  (CALM_CHICK) -  Calmodulin from Gallus gallus

Seq: 149 a.a.

Struc: 151 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DOI no: 10.1002/anie.201302339

Angew Chem Int Ed Engl 52:6246-6249 (2013)

PubMed id: 23630096

A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.

O.V.Moroz, Y.S.Moroz, Y.Wu, A.B.Olsen, H.Cheng, K.L.Mack, J.M.McLaughlin, E.A.Raymond, K.Zhezherya, H.Roder, I.V.Korendovych.

ABSTRACT

It only takes one mutation: A strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, a small, allosterically regulated catalyst of Kemp elimination. In only seven rounds of directed evolution the enzymatic efficiency of the original 74 amino acid residue catalyst was improved more than 220-fold to achieve a kcat value higher than that of catalytic antibodies for the same reaction, still preserving allosteric regulation.