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PDBsum entry 2m3k
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Motor protein
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PDB id
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2m3k
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References listed in PDB file
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Key reference
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Title
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Specific DNA recognition mediated by a type IV pilin.
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Authors
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A.Cehovin,
P.J.Simpson,
M.A.Mcdowell,
D.R.Brown,
R.Noschese,
M.Pallett,
J.Brady,
G.S.Baldwin,
S.M.Lea,
S.J.Matthews,
V.Pelicic.
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Ref.
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Proc Natl Acad Sci U S A, 2013,
110,
3065-3070.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Natural transformation is a dominant force in bacterial evolution by promoting
horizontal gene transfer. This process may have devastating consequences, such
as the spread of antibiotic resistance or the emergence of highly virulent
clones. However, uptake and recombination of foreign DNA are most often
deleterious to competent species. Therefore, model naturally transformable
Gram-negative bacteria, including the human pathogen Neisseria meningitidis,
have evolved means to preferentially take up homotypic DNA containing short and
genus-specific sequence motifs. Despite decades of intense investigations, the
DNA uptake sequence receptor in Neisseria species has remained elusive. We show
here, using a multidisciplinary approach combining biochemistry, molecular
genetics, and structural biology, that meningococcal type IV pili bind DNA
through the minor pilin ComP via an electropositive stripe that is predicted to
be exposed on the filaments surface and that ComP displays an exquisite binding
preference for DNA uptake sequence. Our findings illuminate the earliest step in
natural transformation, reveal an unconventional mechanism for DNA binding, and
suggest that selective DNA uptake is more widespread than previously thought.
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