PDBsum entry 2lhi

Metal binding protein

PDB id: 2lhi

Contents

Protein chain

176 a.a.

Metals

_CA ×3

PDB id:

2lhi

Name:

Metal binding protein

Title:

Solution structure of ca2+/cna1 peptide-bound ycam

Structure:

Calmodulin,serine/threonine-protein phosphatase 2b catalytic subunit a1. Chain: a. Synonym: cam,calcineurin a1,calmodulin-binding protein 1. Engineered: yes. Other_details: the fusion protein of calmodulin (residue 1-146), linker (gsstg) and calcineurin a1 (residues 453-476)

Source:

Saccharomyces cerevisiae (strain atcc 204508 / s288c). Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: cmd1, ybr109c, ybr0904, cmp1, cna1, l9753.6, ylr433c. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

K.Ogura,K.Takahashi,Y.Kobashigawa,R.Yoshida,H.Itoh,M.Yazawa,F.Inagaki

Key ref:

K.Ogura et al. (2012). Solution structures of yeast Saccharomyces cerevisiae calmodulin in calcium- and target peptide-bound states reveal similarities and differences to vertebrate calmodulin. Genes Cells, 17, 159-172. PubMed id: 22280008

Date:

10-Aug-11 Release date: 29-Aug-12

Protein chain

P06787  (CALM_YEAST) -  Calmodulin from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 147 a.a.

Struc: 176 a.a.*

Protein chain

P23287  (PP2B1_YEAST) -  Serine/threonine-protein phosphatase 2B catalytic subunit A1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 553 a.a.

Struc: 176 a.a.*

* PDB and UniProt seqs differ at 146 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.?
• Enzyme class 3: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Genes Cells 17:159-172 (2012)

PubMed id: 22280008

Solution structures of yeast Saccharomyces cerevisiae calmodulin in calcium- and target peptide-bound states reveal similarities and differences to vertebrate calmodulin.

K.Ogura, H.Kumeta, K.Takahasi, Y.Kobashigawa, R.Yoshida, H.Itoh, M.Yazawa, F.Inagaki.

ABSTRACT

No abstract given.