PDBsum entry 2lgg

Ligase/DNA binding protein

PDB id: 2lgg

Contents

Protein chains

69 a.a.

12 a.a.

Metals

_ZN ×3

PDB id:

2lgg

Name:

Ligase/DNA binding protein

Title:

Structure of phd domain of uhrf1 in complex with h3 peptide

Structure:

E3 ubiquitin-protein ligase uhrf1. Chain: a. Fragment: unp residues 298-366. Synonym: inverted ccaat box-binding protein of 90 kda, nuclear protein 95, nuclear zinc finger protein np95, hunp95, ring finger protein 106, transcription factor icbp90, ubiquitin-like phd and ring finger domain-containing protein 1, ubiquitin-like-containing phd and ring finger domains protein 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: uhrf1, icbp90, np95, rnf106. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: the peptide was chemically synthesized.

NMR struc:

20 models

Authors:

C.Wang,J.Shen,Z.Yang,P.Chen,B.Zhao,W.Hu,W.Lan,X.Tong,H.Wu,G.Li,C.Cao

Key ref:

C.Wang et al. (2011). Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger. Cell Res, 21, 1379-1382. PubMed id: 21808299

Date:

26-Jul-11 Release date: 28-Sep-11

Protein chain

Q96T88  (UHRF1_HUMAN) -  E3 ubiquitin-protein ligase UHRF1 from Homo sapiens

Seq: 793 a.a.

Struc: 69 a.a.

Protein chain

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 12 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Cell Res 21:1379-1382 (2011)

PubMed id: 21808299

Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger.

C.Wang, J.Shen, Z.Yang, P.Chen, B.Zhao, W.Hu, W.Lan, X.Tong, H.Wu, G.Li, C.Cao.

ABSTRACT

No abstract given.