PDBsum entry 2lcs

Transferase, signaling protein

PDB id: 2lcs

Contents

Protein chains

67 a.a.

16 a.a.

PDB id:

2lcs

Name:

Transferase, signaling protein

Title:

Yeast nbp2p sh3 domain in complex with a peptide from ste20p

Structure:

Nap1-binding protein 2. Chain: a. Fragment: sh3 domain residues 110-172. Engineered: yes. Serine/threonine-protein kinase ste20. Chain: b. Fragment: sequence database residues 468-483. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: nbp2, ydr162c. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Saccharomyces cerevisiae s288c.

NMR struc:

20 models

Authors:

M.Gorelik,A.R.Davidson

Key ref:

M.Gorelik and A.R.Davidson (2012). Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface. J Biol Chem, 287, 9168-9177. PubMed id: 22277653

Date:

07-May-11 Release date: 01-Feb-12

Protein chain

Q12163  (NBP2_YEAST) -  NAP1-binding protein 2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 236 a.a.

Struc: 67 a.a.*

Protein chain

Q03497  (STE20_YEAST) -  Serine/threonine-protein kinase STE20 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 939 a.a.

Struc: 16 a.a.

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.?
• Enzyme class 2: Chain B: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 287:9168-9177 (2012)

PubMed id: 22277653

Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface.

M.Gorelik, A.R.Davidson.

ABSTRACT

No abstract given.