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PDBsum entry 2l75
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protein metals Protein-protein interface(s) links
Transcription/nuclear protein PDB id
2l75

 

 

 

 

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Contents
Protein chains
56 a.a. *
11 a.a. *
Metals
_ZN ×2
* Residue conservation analysis
PDB id:
2l75
Name: Transcription/nuclear protein
Title: Solution structure of chd4-phd2 in complex with h3k9me3
Structure: Chromodomain-helicase-DNA-binding protein 4. Chain: a. Fragment: phd2 domain, unp residues 446-501. Synonym: chd-4, atp-dependent helicase chd4, mi-2 autoantigen 218 kda protein, mi2-beta. Engineered: yes. 14-meric peptide from 1histone h3.1. Chain: b. Fragment: unp residues 2-14.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: chd4. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: a modified version of pgex-2t containing a hrv protease cleavage site.. Synthetic: yes.
NMR struc: 20 models
Authors: R.E.Mansfield,A.H.Kwan,J.P.Mackay
Key ref: R.E.Mansfield et al. (2011). Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9. J Biol Chem, 286, 11779-11791. PubMed id: 21278251
Date:
02-Dec-10     Release date:   19-Jan-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q14839  (CHD4_HUMAN) -  Chromodomain-helicase-DNA-binding protein 4 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1912 a.a.
56 a.a.
Protein chain
Pfam   ArchSchema ?
P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens
Seq:
Struc: 		136 a.a.
11 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.3.6.4.12  - Dna helicase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O = ADP + phosphate + H+
ATP
 + H2O
 = ADP
 + phosphate
 + H(+)
   Enzyme class 3: Chain B: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
J Biol Chem 286:11779-11791 (2011)
PubMed id: 21278251  
 
 
Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9.
R.E.Mansfield, C.A.Musselman, A.H.Kwan, S.S.Oliver, A.L.Garske, F.Davrazou, J.M.Denu, T.G.Kutateladze, J.P.Mackay.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23211769 C.A.Musselman, M.E.Lalonde, J.Côté, and T.G.Kutateladze (2012).
Perceiving the epigenetic landscape through histone readers.
  Nat Struct Mol Biol, 19, 1218-1227.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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