PDBsum entry 2kyl

Transferase

PDB id: 2kyl

Contents

Protein chains

96 a.a.

13 a.a.

PDB id:

2kyl

Name:

Transferase

Title:

Solution structure of mast2-pdz complexed with thE C-terminus of pten

Structure:

Microtubule-associated serine/threonine-protein kinase 2. Chain: a. Fragment: pdz domain. Engineered: yes. C-terminus of phosphatidylinositol-3,4,5-trisphosphate 3- phosphatase and dual-specificity protein phosphatase pten. Chain: b. Synonym: c-terminus of pten. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic peptide

NMR struc:

10 models

Authors:

E.Terrien,N.Wolff,F.Cordier,C.Simenel,M.Lafon,M.Delepierre

Date:

01-Jun-10 Release date: 08-Jun-11

Protein chain

Q6P0Q8  (MAST2_HUMAN) -  Microtubule-associated serine/threonine-protein kinase 2 from Homo sapiens

Seq: 1798 a.a.

Struc: 96 a.a.*

Protein chain

P60484  (PTEN_HUMAN) -  Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN from Homo sapiens

Seq: 403 a.a.

Struc: 13 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Enzyme class 2: Chain B: E.C.3.1.3.- - ?????
• Enzyme class 3: Chain B: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate
• Enzyme class 4: Chain B: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate
• Enzyme class 5: Chain B: E.C.3.1.3.67 - phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase.

Pathway:

• Reaction: a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5- bisphosphate) + phosphate
• Cofactor: Mg(2+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site