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PDBsum entry 2kfp
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Structural genomics, unknown function
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PDB id
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2kfp
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References listed in PDB file
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Key reference
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Title
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Solution nmr and X-Ray crystal structures of pseudomonas syringae pspto_3016 from protein domain family pf04237 (duf419) adopt a "double wing" DNA binding motif.
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Authors
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E.A.Feldmann,
J.Seetharaman,
T.A.Ramelot,
S.Lew,
L.Zhao,
K.Hamilton,
C.Ciccosanti,
R.Xiao,
T.B.Acton,
J.K.Everett,
L.Tong,
G.T.Montelione,
M.A.Kennedy.
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Ref.
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J Struct Funct Genomics, 2012,
13,
155-162.
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PubMed id
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Abstract
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The protein Pspto_3016 is a 117-residue member of the protein domain family
PF04237 (DUF419), which is to date a functionally uncharacterized family of
proteins. In this report, we describe the structure of Pspto_3016 from
Pseudomonas syringae solved by both solution NMR and X-ray crystallography at
2.5 Å resolution. In both cases, the structure of Pspto_3016 adopts a
"double wing" α/β sandwich fold similar to that of protein YjbR from
Escherichia coli and to the C-terminal DNA binding domain of the MotA
transcription factor (MotCF) from T4 bacteriophage, along with other
uncharacterized proteins. Pspto_3016 was selected by the Protein Structure
Initiative of the National Institutes of Health and the Northeast Structural
Genomics Consortium (NESG ID PsR293).
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