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PDBsum entry 2kbt
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Signaling protein
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PDB id
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2kbt
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References listed in PDB file
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Key reference
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Title
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Attachment of an nmr-Invisible solubility enhancement tag using a sortase-Mediated protein ligation method.
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Authors
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Y.Kobashigawa,
H.Kumeta,
K.Ogura,
F.Inagaki.
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Ref.
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J Biomol Nmr, 2009,
43,
145-150.
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PubMed id
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Abstract
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Sample solubility is essential for structural studies of proteins by solution
NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and
thioredoxin, to a target protein has been used for this purpose. However, signal
overlap of the tag with the target protein often made the spectral analysis
difficult. Here we report a sortase-mediated protein ligation method to
eliminate NMR signals arising from the tag by preparing the isotopically labeled
target protein attached with the non-labeled GB1 tag at the C-terminus.
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